大鼠BMSCs与ADSCs全基因组DNA甲基化差异分析

Comparison of the DNA methylation in a genome wide between BMSCs and ADSCs

目的:探讨BMSCs和ADSCs全基因组甲基化模式。方法:提取大鼠原代培养的BMSCs和ADSCs基因组,使用高通量测序技术检测BMSCs与ADSCs甲基化谱,筛选DNA甲基化差异基因,并对检测结果进行GO分析和Pathway分析。结果:基因组甲基化测序共检测到差异性甲基化区域112 545个,共富集到4 603条GO条目,其中610条GO条目具有显著差异(P<0.05)。通过Pathway分析可以富集到322条通路,其中有98条通路显著富集(P<0.05)。结论:BMSCs与ADSCs基因组存在大量甲基化差异位点,这些甲基化差异位点可能在BMSCs与ADSCs分化与免疫表型的差异有关。

Objective:To investigate the whole genome methylation patterns of BMSCs and ADSCs.Methods:The genomes of the in vitro cultured BMSCs and ADSCs were exacted,the methylation profiles of BMSCs and ADSCs were detected by high-throughput sequencing technology.The DNA methylation difference spectra were screened and the results were analyzed by GO and Pathway analysis.Results:A total of 112 545 differential methylation sites were found by genomic methylation sequencing,a total of 4 603 GO entries were enriched,among which 610 GO entries showed significant differences(P<0.05).322 pathways were identified by pathway analysis,of which 98 were significantly enriched(P<0.05).Conclusion:There are a large number of methylated differences between BMSCs and ADSCs genomes,and these methylated differences may play an important role in the difference between BMSCs and ADSCs differentiation and immune phenotype.