青蒿虎酯对大鼠睾丸扭转复位后缺血再灌注损伤的影响

Effects of artesunate on ischemia-reperfusion injury after testicular torsion/detorsion in rats

目的探讨青蒿虎酯对大鼠睾丸扭转复位后缺血再灌注损伤的影响。方法采用随机数字表法将18只成年健康雄性SD大鼠分为对照组、缺血组和青蒿虎酯组,每组6只,建立睾丸扭转复位动物模型(720°维持2 h)。青蒿虎酯组于术前3 d灌胃青蒿琥酯25 mg/(kg·d),对照组和缺血组灌胃同体积蒸馏水。复位后4 h处死大鼠并获取睾丸组织,分别检测组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)含量,采用HE染色和TUNEL染色检测组织病理学参数和生精细胞凋亡指数(AI)。Western blot检测各组睾丸组织多腺苷二磷酸核糖聚合酶(PARP)-1和凋亡诱导因子(AIF)的表达。结果与对照组比较,缺血组和青蒿虎酯组SOD活性和曲细精管直径明显降低,而MDA含量、凋亡指数、PARP-1和AIF表达则显著升高,差异有统计学意义(P <0.05);与缺血组比较,青蒿虎酯组SOD活性和曲细精管直径显著升高,MDA含量、凋亡指数、PARP-1和AIF表达明显降低,差异有统计学意义(P <0.05)。结论青蒿虎酯对睾丸扭转复位后的缺血再灌注损伤具有保护作用,其机制可能是通过抑制细胞凋亡和氧化应激表达而实现。

Objective To investigate the effects of artesunate on ischemia-reperfusion injury after testicular torsion/detorsion in rats. Methods Eighteen adult healthy male Sprague Dawley rats were divided into control group, ischemia group and artesunate group by random number table method, with 6 rats in each group. The model rats with testicular torsion/detorsion were made (720° for 2 h). The artesunate group was given artesunate 25 mg/(kg·d) by gavage administration at 3 d before operation. The control group and ischemia group were given same volume of distilled water by gavage administration. After detorsion for 4 h, the rats were sacrificed and the testicular tissue was obtained, the contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were detected, and the histopathological parameters and apoptosis index (AI) were tested by HE staining and TUNEL staining. The expression of poly (ADP-ribose) polymerase (PARP)-1 and apoptosis-inducing factor (AIF) in testicular tissue of each group was detected by Western blot. Results Compared with control group, the SOD activity and the diameter of seminiferous tubules in ischemia group and artesunate group were significantly decreased, while the MDA content, apoptosis index, the expression of PARP-1 and AIF in ischemia group and artesunate group were significantly increased, the differences were statistically significant (P < 0.05). Compared with ischemia group, the SOD activity and the diameter of seminiferous tubules in artesunate group were significantly increased, while the MDA content, apoptosis index, the expression of PARP-1 and AIF in artesunate group were significantly decreased, the differences were statistically significant (P < 0.05). Conclusion Artesunate has protective effects on ischemia-reperfusion injury after testicular torsion/detorsion, its mechanism may be achieved through inhibiting apoptosis and oxidative stress.