东亚小金发藓中1个新的二苯甲酮类化合物

A new benzophenone from endohydric moss Pogonatum inflexum

目的对东亚小金发藓Pogonatum inflexum全草的化学成分进行研究,以期发现新的活性化合物。方法采用硅胶、凝胶、MCI-Gel树脂及RP-HPLC等多种色谱技术进行分离纯化,运用波谱方法对所分离的化合物进行结构鉴定。并用MTT法测定了化合物1对Hep G2、HL-60、A549和MCF-7 4种人源肿瘤细胞株的细胞毒活性。结果从东亚小金发藓全草丙酮提取物中分离得到2个化合物,分别鉴定为2-(4-苯甲酰基-3-羟基苯氧基)-1-(哌啶-1-基)乙-1-酮(1)、2-(4-苯甲酰基-3-羟基苯氧基)乙酸叔丁酯(2)。结论化合物1为新化合物,命名为金发藓酮A;化合物2为首次从金发藓科植物中分离得到;化合物1对人肝癌细胞HepG2具有较强的细胞毒活性。

Objective To study the chemical components of endohydric moss Pogonatum inflexum in order to find new bioactive compounds. Methods The constituents were separated by column chromatographic methods of silica gel, MCI-Gel resin, Sephadex LH-20, and HPLC. The structures were elucidated by spectroscopic data analysis. The cytotoxicity of compounds for HepG2, HL-60,A549, and MCF7 cell line was also evaluated by using MTT method. Results Two compounds were isolated and identified as2-(4-benzoyl-3-hydroxyphenoxy)-1-(piperidin-1-yl) ethan-1-one(1) and tert-butyl-2-(4-benzoyl-3-hydroxyphenoxy) acetate(2).Conclusion Compounds 1 is a new compound named pogonatone A;Compound 2 is isolated from Polytrichaceae species for the first time;Compound 1 also displays the high cytotoxicity to HepG2 cell line.