植物激素及木质素对牛樟芝菌丝体生长情况的影响

Effects of phytohormones and lignin on growth of Antrodia cinnamomea mycelium

目的探究植物激素及木质素对牛樟芝菌丝体生长情况的影响,并对其培养产物进行粗多糖、粗三萜含量及抗氧化活性测定研究。方法采用皿式培养法培育牛樟芝,超声提取培养产物,选用苯酚-硫酸法测定粗多糖含量、香草醛-冰醋酸法测定粗三萜含量,并以DPPH自由基、ABTS自由基的半数清除浓度(IC_(50))作为培养产物抗氧化活性的评价指标。结果菌种活化方法为培养20 d后靠近菌丝层边缘取样,可获得生长活力好的接种原料;改良PCA培养基上牛樟芝菌丝长势较好、粗三萜及粗多糖含量高;以改良PCA培养基为基础,向其中添加0.5g/L木质素时菌丝生长最快,菌丝层直径为对照组的1.19倍;当IBA添加质量浓度为0.5 mg/L时,菌丝体干质量较对照组增加了89.51%,粗多糖产量较对照组提升了130.57%,远高于其他组别,与此同时,菌丝体粗三萜含量及产量也显著提升,其含量较对照组提高了61.31%,粗三萜产量达到133.24mg/L;添加牛樟树粉末0.5 g/L时,菌丝体粗三萜含量最高,为5.62%,较对照组提升了84.26%;此外,向培养基中加入植物激素及木质素对牛樟芝菌丝体的抗氧化能力提升有一定作用,整体来说加入不同物质后实验组抗氧化活性良好。结论通过向改良PCA培养基中添加植物激素及木质素,可有效促进牛樟芝菌丝体生长、提高活性成分含量并提升其抗氧化活性。

Objective To investigate the effects of phytohormones and lignin on the growth of Antrodia cinnamomea mycelium, and to determine the content of crude polysaccharide and crude triterpenoid and antioxidant activity of cultured products. Methods The product cultured by solid-state fermentation in petri dish was ultrasonically extracted. The phenol-sulfuric acid method was selected to determine the content of crude polysaccharide of the extract, and the vanillin-glacial acetic acid method was used to determine the content of crude triterpenoids of the extract. The half-clearing concentrations(IC50) of DPPH free radicals and ABTS free radicals were indexes for evaluating the anti-oxidant activity of the culture product. Results Sampling near the outer edge of the mycelium layer after 20 d of culture was performed by the activation method to obtain inoculated raw materials with better growth activity;The basal medium with good growth, high content of crude polysaccharide and crude triterpenoids was modified PCA medium;On the basis of this medium, the mycelia with 0.5 g/L lignin added into the medium grew fastest, and the diameter of mycelium layer increased to1.19 times of the control group;When the concentration of IBA was 0.5 mg/L, the dry weight of mycelium was increased by 89.51%compared with the control group, and the yield of crude polysaccharide was increased by 130.57% compared with the control group,which was much higher than other groups. The content and yield of triterpenoids were also increased significantly, 61.31% higher than the control group, and crude triterpenoids yield reached 133.24 mg/L;The content of crude triterpenoids in mycelium was the highest(5.62%) when adding 0.5 g/L powder of Cinnamomum kanehirai, which was 84.26% higher than that of the control group;In addition, the addition of plant hormones and lignin to the culture medium has a certain effect on the anti-oxidant capacity of the Antrodia cinnamomea mycelium, on the whole, the experimental group had good anti-oxidant activity after adding different substances. Conclusion By adding phytohormone and lignin to the modified PCA medium, the growth of the mycelium of Antrodia cinnamomea can be effectively promoted, the content of the active ingredient can be increased, and the anti-oxidant activity can be enhanced.