一测多评法同时测定复生康胶囊中8个成分的含量

Simultaneous determination of eight components in Fushengkang capsule by QAMS

目的:采用一测多评法同时测定复生康胶囊中羟喜树碱、喜树碱、荭草苷、异荭草苷、牡荆苷、异牡荆苷、莪术二酮和莪术醇的含量。方法:色谱柱为Hypersil ODS柱(4.6 mm×250 mm,5μm);以甲醇-乙腈(2∶1)与0.05%冰醋酸溶液为流动相,梯度洗脱;柱温为25℃;检测波长分别为254 nm(检测羟喜树碱和喜树碱)、340 nm(检测荭草苷、异荭草苷、牡荆苷和异牡荆苷)、214 nm(检测莪术二酮和莪术醇);体积流量为0.9 mL·min^(-1);以牡荆苷为内标物,建立牡荆苷与羟喜树碱、喜树碱、荭草苷、异荭草苷、异牡荆苷、莪术二酮和莪术醇的相对校正因子,测定其含量。结果:羟喜树碱、喜树碱、荭草苷、异荭草苷、牡荆苷、异牡荆苷、莪术二酮和莪术醇分别在2.69～67.25、3.88～97.00、1.46～36.50、1.77～44.25、1.19～29.75、1.85～46.25、2.09～52.25、1.73～43.25μg·mL^(-1)范围内线性关系良好;平均加样回收率(RSD)分别为99.1%(0.91%)、100.1%(0.67%)、97.9%(1.2%)、98.2%(1.4%)、97.5%(0.97%)、97.0%(1.4%)、98.9%(0.89%)和98.1%(1.3%);一测多评法所得结果与外标法无显著差异。结论:一测多评法可以应用于复生康胶囊的多指标质量评价。

Objective:To establish a method for simultaneous determination of hydroxycamptothecin, camptothecine,orientin,isoorientin,vitexin,isovitexin,curdione and curcumol in Fushengkang capsule by multi-components by single-marker(QAMS) method.Methods:The analysis was performed on a Hypersil ODS column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of methanol-acetonitrile(2∶1)(A)-0.05% glacial aceticacid solution(B) flowing at 0.9 mL·min-1 in a gradient elution.The column temperature was set at 25 ℃,and the detection wavelengths were set at 254 nm(hydroxycamptothecin and camptothecine),340 nm(orientin, isoorientin,vitexin and isovitexin) and 214 nm(curdione and curcumol).With vitexin as the internal standard, the relative correction factors of hydroxycamptothecin,camptothecine,orientin, isoorientin,isovitexin,curdione and curcumol were calculated, based on which the contents were determined.Results:The standard curves of hydroxycamptothecin,camptothecine,orientin, isoorientin,vitexin,isovitexin,curdione and curcumol showed good linearity in 2.69-67.25,3.88-97.00,1.46-36.50,1.77-44.25,1.19-29.75,1.85-46.25,2.09-52.25 and1.73-43.25 μg·mL-1,respectively.The average recoveries and the corresponding RSD were 99.13%(0.91%),100.1%(0.67%),97.9%(1.2%),98.2%(1.4%),97.5%(0.97%),97.0%(1.4%),98.9%(0.89%) and 98.1%(1.3%),respectively.The results obtained by QAMS approximated with those obtained by external standard method.Conclusion:The QAMS method can be used to the multi-index quality evaluation of Fushengkang capsule.