摘要
Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin extraction method from rapeseed meal(RSM) was explored. Effects of hydrochloric acid(HCl) concentration, ethanol concentration, extraction temperature and time, and solid-liquid ratio were evaluated systematically. According to results of single factor experiment and Box-Behnken experiment, the optimum extraction conditions of melanin from RSM were as follows: HCl concentration, 0.5 mol/L; ethanol concentration, 70%; extraction temperature, 40 o C; extraction time, 1 h; solid liquid ratio, 1/4 g/mL. Under these conditions, extraction yields of crude melanin(CM) and purified melanin(PM) were 9.00% and 1.44%, respectively. Compared with synthetic melanin(SM) from Sigma, the relative purities of CM and PM were 7.82% and 29.57%, respectively. Moreover, feedstuff value of RSM residue was also improved after melanin isolation by significantly reducing glucosinolate content.
Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin extraction method from rapeseed meal(RSM) was explored. Effects of hydrochloric acid(HCl) concentration, ethanol concentration, extraction temperature and time, and solid-liquid ratio were evaluated systematically. According to results of single factor experiment and Box-Behnken experiment, the optimum extraction conditions of melanin from RSM were as follows: HCl concentration, 0.5 mol/L; ethanol concentration, 70%; extraction temperature, 40 o C; extraction time, 1 h; solid liquid ratio, 1/4 g/mL. Under these conditions, extraction yields of crude melanin(CM) and purified melanin(PM) were 9.00% and 1.44%, respectively. Compared with synthetic melanin(SM) from Sigma, the relative purities of CM and PM were 7.82% and 29.57%, respectively. Moreover, feedstuff value of RSM residue was also improved after melanin isolation by significantly reducing glucosinolate content.
基金
supported by Ministry of Science and Technology of the People’s Republic of China (2016YFD0501209)
Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2013-OCRI)
