不同来源百合药材的质量评价及分析

Quality Evaluation and Analysis of Lilii Bulbus from Different Sources

目的基于水分、浸出物、多糖、Regaloside A含量进行不同来源百合药材质量评价及分析,为多基原百合药材鉴别及质量评价提供依据。方法按照2015版《中华人民共和国药典》方法测定水分、浸出物;采用紫外分光光度法测定百合多糖含量;采用高效液相色谱法测定Regaloside A含量:Supersil ODS-B(250 mm×4.6 mm, 5μm)色谱柱,柱温为30℃,进样量为10μL,流速为1.0 mL/min,流动相0.1%磷酸水溶液∶乙腈=85∶15(V/V),检测波长为310 nm。结果 54批百合样品水分均≤13%;43批百合样品浸出物≥18%,11批百合样品浸出物<18%;54批百合样品多糖含量区间为9.89%～23.42%;54批百合样品Regaloside A含量区间为0.019%～0.599%。结论所有百合样品水分均符合药典要求;11批百合药材浸出物未达到药典要求,且均为龙牙百合药材;17批百合多糖含量未达湖南省地方标准;卷丹百合浸出物和Regaloside A含量均普遍高于市购龙牙百合药材。该研究为市场药用百合以卷丹百合为主提供了佐证,为不同来源百合鉴别及百合药材质量控制提供了参考依据。

Objective To evaluate and analyze the quality of Lilii Bulbus from different sources by the contents of moisture, bulbus extract, polysaccharides and Regaloside A, so as to provide the basis for identification and quality control of polybasic lilium. Methods Moisture and bulbus extract were determined according to the 2015 edition of Chinese Pharmacopoeia. Polysaccharides was determined by ultraviolet spectrophotometry. Regaloside A was determined by high performance liquid chromatography. The column was a Supersil ODS-B column (250 mm×4.60 mm, 5 μm). The column emperature was 30℃, and the injection volume was 10 μL. The flow rate was 1.0 mL/min, the mobile phase 0.1% phosphoric acid aqueous solution-acetonitrile volume ratio of 85:15 (V/V). The detection wavelength was at 310 nm. Results The moisture contents of 54 batches of Lilii Bulbus were all less than 13%. The bulbus extracts of 43 batches of Lilii Bulbus were greater than 18%, and the bulbus extracts of the other 11 batches of Lilii Bulbus were less than 18%. The polysaccharidse contents of 54 batches of Lilii Bulbus range from 9.89% to 23.42%. The Regaloside A content range of 54 batches of Lilii Bulbus was 0.019%~0.599%. Conclusion The moisture of 54 batches of Lilii Bulbus all meets the requirements of Chinese Pharmacopoeia. The bulbus extract of 11 batches of Lilii Bulbus were unqualified, which are all from Long Ya Lilii Bulbus. The mean polysaccharide content of Lilii Bulbus from different sources was not significantly different. The bulbus extract and Regaloside A contents of Juan dan Lilii Bulbus were generally higher than those of Long Ya Lilii Bulbus on the market, which provided evidence for the fact that Juan Dan Lilii Bulbus was the main medicinal material in the market. This study can provide more references for identification and quality control of Lilii Bulbus from different sources.