摘要
研究了大孔吸附树脂分离纯化棘白霉素B母核(ECBN)的工艺条件。以大孔吸附树脂D303为脱色树脂、HZ835为吸附树脂,将ECBN滤液pH值调至5.5进行吸附,吸附量为14 512μg·mL^(-1);再以5%乙醇水溶液为解吸剂解吸,解吸率为91.8%;最后经纳滤浓缩、脱炭、结晶、真空干燥等工艺得到ECBN粗粉,含量为65%左右,色谱纯度大于80%,提取总收率超过60%。该工艺简单易行、操作成本低、溶媒消耗少,适合工业化生产。
We studied the separation and purification conditions of Echinocandin B nucleus (ECBN) by macroporous adsorption resin.Using resin D303 as a decolorization resin,resin HZ835 as an adsorption resin,and 5% ethanol aqueous solution as a desorbent,the ECBN (pH=5.5) is dynamically adsorbed and desorbed with an adsorption capacity of 14 512 μg·mL ^-1 and a desorption rate of 91.8%,respectively.Then,through nanofiltration concentration,decarbonization,crystallization,and vacuum drying,we obtained ECBN crude powder with the content of about 65%,the purity of more than 80%,and the total yield of more than 60%.The process has the advantages of simple operation,low cost,low solvent consumption,which is suitable for industrial production.
作者
彭亮
王晶珂
谢新宇
李敏
PENG Liang;WANG Jingke;XIE Xinyu;LI Min(National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering & Technology Research Center,New Drug Research and Development Co.,Ltd.,North China Pharmaceutical Corporation,Shijiazhuang 050015,China)
出处
《化学与生物工程》
CAS
2019年第4期10-12,23,共4页
Chemistry & Bioengineering
基金
国家"重大新药创制"科技专项(2014ZX09201001-002)
关键词
棘白霉素B母核
大孔吸附树脂
分离纯化
Echinocandin B nucleus
macroporous adsorptionresin
separation and purification