大孔吸附树脂分离纯化棘白霉素B母核的工艺研究

Separation and Purification of Echinocandin B Nucleus by Macroporous Adsorption Resin

研究了大孔吸附树脂分离纯化棘白霉素B母核(ECBN)的工艺条件。以大孔吸附树脂D303为脱色树脂、HZ835为吸附树脂,将ECBN滤液pH值调至5.5进行吸附,吸附量为14 512μg·mL^(-1);再以5%乙醇水溶液为解吸剂解吸,解吸率为91.8%;最后经纳滤浓缩、脱炭、结晶、真空干燥等工艺得到ECBN粗粉,含量为65%左右,色谱纯度大于80%,提取总收率超过60%。该工艺简单易行、操作成本低、溶媒消耗少,适合工业化生产。

We studied the separation and purification conditions of Echinocandin B nucleus (ECBN) by macroporous adsorption resin.Using resin D303 as a decolorization resin,resin HZ835 as an adsorption resin,and 5% ethanol aqueous solution as a desorbent,the ECBN (pH=5.5) is dynamically adsorbed and desorbed with an adsorption capacity of 14 512 μg·mL ^-1 and a desorption rate of 91.8%,respectively.Then,through nanofiltration concentration,decarbonization,crystallization,and vacuum drying,we obtained ECBN crude powder with the content of about 65%,the purity of more than 80%,and the total yield of more than 60%.The process has the advantages of simple operation,low cost,low solvent consumption,which is suitable for industrial production.