聚乙烯醇/纳米羟基磷灰石/丝素蛋白复合水凝胶的制备及其性能研究

Preparation and properties of polyvinyl alcohol/nano-hydroxyapatite/silk fibroin composite hydrogel

本文以聚乙烯醇(PVA)、纳米羟基磷灰石(n-HA)和丝素蛋白(SF)为原料,采用物理共混法、反复冷冻解冻法和NaCl粒子制孔法制得了具有三维结构的PVA/n-HA/SF多孔复合水凝胶,以作为人工角膜支架材料。测试了这种多孔复合材料的含水率、拉伸强度和断裂伸长率,并对其进行了红外谱图、X射线衍射光谱、热重及电子扫描显微镜分析。结果表明,丝素蛋白的添加量增加时,多孔复合水凝胶的含水率相应提高,含水率稳定在75～82%之间;其拉伸强度在0.43～1.00MPa之间,断裂伸长率在183.76～237.53%之间,可以达到人体正常状态下眼压要求,其中复合水凝胶的最佳配比为:PVA:SF:n-HA=10:5:1。;IR和XRD分析表明复合水凝胶在物理交联过程中,各种成分均匀复合,无化学键变化;扫描电镜显示该水凝胶材料具有均匀的三维多孔结构。

The PVA/n-HA/SF porous composite hydrogel as an artificial cornea scaffold was prepared by using polyvinyl alcohol(PVA),nano-hydroxyapatite(n-HA)and silk fibroin(SF)via the physical blending method,repeated freezing and thawing method and pore method of NaCl particles.The properties of the composite were tested by mechanical measurements of the moisture content,the tensile strength and elongation at break.and the morphology and structure of the composite was characterized by Infrared Spectroscopy(IR),X-ray Diffraction Spectrum(XRD),Thermogravimetric analysis(TG)and Scanning Electron Microscope(SEM).The results show that moisture content of the composite hydrogel are among 75~82%;tensile strength and breaking elongation are at a range of 0.43~1.00MPa and 183.76%~237.53%respectively,which meets the demand of human intraocular pressure;Infrared spectrogram(IR)and X-ray diffraction(XRD)indicated that in the course of physical cross-linking of composite hydrogels,the three raw materials have been induced in the composite and there were no chemical reactions;Scanning electron microscope(SEM)demonstrates a three-dimensional homogeneous distributed porous structure of composite hydrogels which is an ideal structure for cell adhesion and nutrient transport and metabolic product transportation.