重瓣榆叶梅茎段组织培养体系的建立

Establishment of tissue culture system of stem segment in Amygdalus triloba f. multiplex

以重瓣榆叶梅带腋芽的茎段作为试验材料,研究了不同外植体消毒方式、初代培养基激素配比和生根培养基激素配比对重瓣榆叶梅组织培养的影响。结果表明:1)先用75%酒精处理30 s,再使用0.1%升汞溶液消毒8 min或以2%次氯酸钠溶液消毒12 min,外植体感染率最低;2)茎段初代培养最佳培养基为MS+6-BA2.0 mg/L+NAA 0.05 mg/L,诱导率为89.30%;3)最佳增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+KT1.0 mg/L,增殖倍数为4.4,且生长状况良好;4)1/2MS+IBA 0.2 mg/L为最佳生根培养基;5)该研究建立了重瓣榆叶梅组培再生体系,为工厂化育苗与大面积推广奠定了基础。

The stem segments with the axillary buds of Amygdalus triloba f. multiplex were used as test materials. The effects of different explants disinfection ways, primary culture medium hormone ratio and rooting medium hormone ratio on tissue culture of A. triloba were studied. The results show that: 1) Firstly the explant was treated with 75% alcohol for 30 s, then disinfected with 0.1% mercuric chloride solution for 8 min or disinfected with 2% sodium hypochlorite solution for 12 min, the explant infection rate was the lowest;2) The optimal medium for primary culture of stem segments was MS + 6-BA 2.0 mg/L + NAA 0.05 mg/L, and the induction rate was 89.30%;3) The optimal proliferation medium was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L + KT 1.0 mg/L, the proliferation multiple was 4.4, and the growth condition was good;4) 1/2MS + IBA 0.2 mg/L medium was the best rooting medium;5) This study established a tissue culture regeneration system of A. triloba, which laid a foundation for planting seedlings and large-scale promotion.