摘要
以毛泡桐无菌试管苗为试验材料,研究了基本培养基、激素、糖及浓度对毛泡桐组培苗不定芽增殖及生长的影响,结果表明:基本培养基、激素的种类不同导致增殖培养结果存在很大差异,在基本培养基和激素组合增殖培养中,以WPM和BAP相结合是最适宜的培养基。在调查糖的种类对增殖培养的影响中,还是以蔗糖效果最好。不同蔗糖浓度的培养试验中,随着糖浓度的增加,其增殖诱导数增多,但小苗的伸长在20 g·L^(-1)区间增长显著。
The effect of basic medium,plant hormone,different sugar with varied concentration on proliferation and growth of adventitious bud was studied in this paper taking tissue culture seedling of Paulownia tomentosa as experiment material.It showed that different basic medium,hormone resulted in varied proliferation effect.WPM+BAP was optimum culture medium,and sucrose was optimum sugar.Proliferation number increased with growing sugar concentration,and 20 g·L^-1 resulted in maximum height growth.
作者
蒋祥娥
蔡桁
田玲
管兰华
佐佐木义则
Jiang Xiange;Cai Heng;Tian Ling;Guan lanhua;Yoshinori Sasaki(Forest Tree seed and Seedling Administrative General Station of Hebei Forestry Department Wuhan 430079;Jingmen Peng Chang forest farm Jingmen 448269;Oita forest experimental site, oita prefecture, Japan 8770023)
出处
《湖北林业科技》
2019年第2期4-6,共3页
Hubei Forestry Science and Technology
关键词
培养基
植物激素
糖种类和浓度
毛泡桐
组培增殖
medium
plant hormone
different sugar with varied concentration
Paulownia tomentosa
tissue culture
