摘要
以崖柏茎段为外植体,MS为基本培养基,探讨0.1%升汞不同消毒时间对外植体消毒效果;不同盐浓度的培养基对外植体生长的影响。结果表明:0.1%升汞消毒12 min污染率最低,杀伤率低,表面灭菌效果最好;1/2MS培养基上培养愈伤组织长势效果最好。
In Thuja sutchuenensis stem segments as explants,MS as the basic culture medium,To investigate the effects of different sterilization time of 0.1%HgCl2 explant disinfection effect;medium different salt concentration outside the plant growth.The results showed:The 0.1%Hgcl2 disinfection 12min pollution rate is the lowest,the killing rate is low,the surface sterilization effect is best;the 1/2MS medium culture callus growth effect is the best.
作者
王毅敏
高晗
高本旺
宋正江
陈发菊
Wang Yimin;Gao Han;Gao Benwang;Song Zhengjiang;Chen Faju(The Three Gorges Botanical Garden Yichang 443111;China Three Gorges University Yichang 443000)
出处
《湖北林业科技》
2019年第2期16-18,共3页
Hubei Forestry Science and Technology
关键词
崖柏
研究进展
消毒
组织培养
无菌系
Thuja sutchuenensis
research progress
disinfection
tissue culture
sterile lines
