葛根素改善卒中后抑郁大鼠行为学的研究

Study of puerarin on behaviour of rats with post-stroke depression

目的观察葛根素对卒中后抑郁(PSD)大鼠行为学的影响。方法采用大脑中动脉栓塞法建立局灶性脑缺血再灌注损伤大鼠模型,采用慢性不可预见温和应激性刺激(CUMS)联合孤养法建立PSD模型。将雄性SD大鼠分为对照组、PSD+生理盐水组(PSD组)及PSD+葛根素组(PSD+Pue组),每组8只。PSD+Pue组及PSD组在应激刺激开始时给予腹腔注射葛根素(100mg/kg)或等体积生理盐水1周。比较三组大鼠行为学及脑梗死体积。结果应激后,PSD组大鼠体重、SP、水平及垂直运动次数均较对照组大鼠显著下降(P<0.05);PSD+Pue组上述水平均优于PSD组(P<0.05)。PSD组及PSD+Pue组大鼠的脑梗死体积大于对照组,但PSD+Pue组小于PSD组(P<0.05)。结论葛根素能改善PSD大鼠的抑郁行为,并且这种作用与其脑保护作用一致。

Objective To investigate the influence of puerarin on behaviour of rats with post-stroke depression (PSD). Methods Focal cerebral ischemia-reperfusion injury model in rats were established by middle cerebral artery embolization,and PSD model were established by chronic unpredictable mild stress stimulation (CUMS) combined with orphanage. Male SD rats were divided into control group, PSD+saline group (PSD group) and PSD+puerarin group (PSD+Pue group), with 8 rats in each group. Puerarin (100 mg/kg) or normal saline were injected intraperitoneally for one week at the beginning of stress stimulation respectively in PSD+Pue group and PSD group. Behavior and cerebral infarction volume of rats in the three groups were compared. Results After stress, the body weight, SP and the times of horizontal and vertical movements of rats in the PSD group were lower than those in the control group (P<0.05), and those in the PSD+Pue group were better than the PSD group(P<0.05). The volume of cerebral infarction in the PSD group and PSD+Pue group were larger than that in the control group, but that in the PSD+Pue group was smaller than that in the PSD group (P<0.05). Conclusion Puerarin can attenuate depression-like behavior in PSD rat, and the protective effect is related with brain protection.