灵龟八法对衰老大鼠IL-10、IFN-γ活性及基因表达的影响

Effect of Eight Methods of Intelligent Turtle on the Activity and Gene Expressions of IL-10 and INF-Y in Aging Rats

目的:观察灵龟八法按时开穴灸对衰老模型大鼠脾脏组织中IL-10和IFN-γ活性及IL-10mRNA、IFN-γmRNA基因表达的影响。方法:把实验大鼠随机分为空白组、模型组、灵龟八法治疗组和脾俞加肾俞治疗组,共4组。采用D-半乳糖造模,建立大鼠亚急性衰老模型,用Elisa法观察大鼠脾脏组织IL-10和IFN-γ的活性变化,用RT-PCR法检测大鼠脾脏组织IL-10 mRNA、IFN-γmRNA基因表达。结果:灵龟八法治疗组IL-10活性及IL-10 mRNA基因表达量明显高于模型组(P <0. 05),与空白组相比下降,差异有统计学意义(P <0. 05);灵龟八法治疗组IFN-γ活性及IFN-γmRNA基因表达量明显低于模型组(P <0. 05),与空白组相比上升(P <0. 05),差异有统计学意义。结论:灵龟八法开穴灸可有效抑制致炎因子IFN-γ、IFN-γmRNA的生成,增加抗炎因子IL-10、IL-10 mRNA的数目,减轻D-半乳糖引起的炎症反应并恢复机体的抗炎平衡,加强对脾脏细胞的保护,起到延缓衰老的作用,证明了灵龟八法延缓衰老的机理与调节免疫机制密切相关。

Objective:To observe the effect of eight methods of intelligent turtle on the activity and gene expressions of IL-10 and IFN-γ in the spleen tissues of aging rats. Methods:Experimental rats were randomly divided into the blank group,the model group,the treatment group,and the control group.A model of subacute aging model was established by using D-galactose in rats.The treatment group was given moxibustion with eight methods of intelligent turtle,and the control group was given moxibustion on Pishu(BL20) and Shenshu(BL23).The activities of IL-10 and IFN-γ in the spleen tissues were observed by Elisa method.The gene expressions of IL-10 mRNA and IFN-γ mRAN were detected by PCR technique. Results:The activity of IL-10 and the gene expression of IL-10 mRNA were significantly higher in the treatment group than those in the model group( P <0.05);they were lowered in the treatment group when compared to the blank group( P <0.05).The activity of IFN-γ and the gene expression of IFN-γ mRNA were significantly decreased in the treatment group compared to those in the model group( P <0.05);they were increased in the treatment group when compared to the blank group( P <0.05). Conclusion:Eight methods of intelligent turtle can effectively inhibit the production of inflammatory agent of IFN-γ,increase the number of anti-inflammatory agent of IL-10,reduce the inflammatory reaction caused by D-galactose and restore the body anti-inflammatory balance,as well as strengthen the protection of spleen cells.It plays an important role in delaying senility.