水牛发情周期生殖激素变化规律及唾液结晶的分析

The Variations and Correlation Analysis of Saliva Reproduction Hormones and Crystallization Patterns During Estrus Cycle in Buffaloes

试验对水牛发情周期血清和唾液中雌二醇(E_2)和孕酮(P_4)的浓度变化规律、水牛唾液结晶与卵泡发育变化分别进行了分析研究,为进一步探讨水牛发情规律、指导生产提供依据。采用酶联免疫分析法(ELISA)测定发情母水牛血清和唾液中E_2和P_4的浓度变化,并对血清和唾液的激素变化规律进行相关性分析。结果表明,水牛血清和唾液中的E_2和P_4呈波动性变化。发情前期,唾液中P_4浓度一直维持在6.50～7.10 ng/mL,发情第13天达到11.09 ng/mL,随后快速下降。唾液中E_2浓度在发情第3～5天出现一个峰值178.53 pg/mL,在第14～17天唾液中E_2浓度显著升高,出现第二个峰值179.10 pg/mL。母水牛唾液中E_2和P_4浓度的变化趋势与其在血清中的变化趋势基本一致,均呈显著相关(P<0.05);唾液中E_2与P_4浓度呈极显著相关(P<0.01)。水牛发情当天唾液结晶呈现明显的蕨类作物形状且分维值显著低于其他时间点(P<0.05)。水牛发情周期唾液结晶图形的变化与卵巢卵泡发育基本同步,可作为监测水牛发情及预测排卵的可靠指标之一。

The aim of the experiment was to explore the buffalo estrus and provide theoretical references for buffalo production by studying the change laws of estradiol(E2)and progesterone(P4)concentrations during estrus cycle of salivary and serum,investigating the salivary ferning and the development of ovaries in buffalo.The concentrations of E2 and P4 were determined by the enzyme linked immunosorbent assay(ELISA)in estrus cycle,and the correlation between the serum and saliva hormones were analyzed.The results show that the concentrations of E 2 and P4 varied fluctuantly.The concentration of saliva P 4 was maintained from 6.50 to 7.10 ng/mL at the beginning of estrus,and reached 11.09 ng/mL at 13 d of estrus,then fell fast.The concentration of saliva E2 had two peaks,respectively at 3-5 d(178.53 pg/mL)and 14-17 d(179.10 pg/mL)of estrus.The change trends of E2 and P 4 levels in salivary and serum had the same change trends,the concentrations of salivary P4 and E2 had significant correlations with that in serum(P<0.05),while the salivary E2 concentration also had a extremely significant correlation with the salivary P4 concentration(P<0.01).Saliva showed a typical fern-like crystallization patterns at 0 d of estrus,with significantly lower fractal dimension values compared with the other days of estrus(P<0.05).The results were also showed the basic synchronization between saliva crystallization and ovarian follicle.In conclusion,saliva crystallization could be used as a reliable index for monitoring estrus and predicting ovulation in buffalo.