摘要
CRISPR/dCas9是以CRISPR/Cas9系统为基础发展而来的转录调控工具.本文综述了CRISPR/dCas9作为转录抑制工具(CRISPRi)和转录激活工具(CRISPRa)的发展及应用,总结了在细菌中应用CRISPR/dCas9系统时转录调控系统的选择、构建、靶位点的选择以及gRNA的设计等方面存在的问题,并对相关解决方法进行了展望.
CRISPR/dCas9 is a transcriptional regulation tool developed from the CRISPR/Cas9 system. The development and application of CRISPR/dCas9 as a transcriptional interference(CRISPRi)and transcription activator(CRISPRa)were summarized in this paper. Problems in the application of CRISPR/dCas9 system in bacteria, such as target sites, target selection and construction of the transcriptional regulatory system, the design of gRNA, and possible solutions were also discussed in the paper.
作者
钟成
黄龙辉
刘其敬
谢燕燕
谭之磊
ZHONG Cheng;HUANG Longhui;LIU Qijing;XIE Yanyan;TAN Zhilei(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China)
出处
《天津科技大学学报》
CAS
2019年第2期1-5,11,共6页
Journal of Tianjin University of Science & Technology
基金
国家自然科学基金资助项目(31470610
21576212)
