HDAC抑制剂SAHA抑制人乳腺癌细胞MCF-7的迁移和增殖

Inhibiting the Proliferation and Migration of Human Breast Cancer Cell MCF-7 with HDAC Inhibitor SAHA

用组蛋白去乙酰化酶(histone deacetylase complex,HDAC)抑制剂SAHA对人乳腺癌细胞MCF-7进行处理,抑制组蛋白的去乙酰化,进而检测SAHA对MCF-7的影响.体外划痕实验与Transwell实验表明SAHA可以抑制MCF-7细胞的迁移;免疫印迹(Western blot)实验表明SAHA可以抑制迁移相关基因MYL9蛋白水平的表达.MTT实验和克隆形成实验表明SAHA可以抑制MCF-7细胞的增殖;Western blot实验与逆转录实时荧光定量PCR(RT-qPCR)实验表明SAHA能够抑制细胞周期蛋白标志基因CyclinD1、CDK4的表达,促进周期蛋白激酶抑制剂p21的表达.

Histone deacetylase complex(HDAC)inhibitor SAHA was used to deal with human breast cancer cell MCF-7 and inhibit the deacetylation of histone proteins in order to detect the effects of SAHA on MCF-7 cells. Wound healing assays and transwell assays showed that SAHA inhibited the migration of MCF-7 cells. Western blot assays proved that SAHA inhibited the expression of migration-related gene MYL9. MTT assays and colony forming assays demonstrated that SAHA inhibited the proliferation of MCF-7 cells. Western blot assays and RT-qPCR assays proved SAHA inhibited the expression of cell cycle marker genes CyclinD1 and CDK4 and promoted the expression of CDK inhibitor p21.