摘要
[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi. [Methods] The contents of chlorophyll(Chl) and soluble sugar were detected with ultraviolet spectrophotometry. The activities of phenylalanine ammonia-lyase(PAL), cinnamate 4-hydroxylase(C_4H) and chalcone synthase(CHS) were determined with ultraviolet spectrophotometry. Secondary metabolites were detected by high performance liquid chromatography(HPLC). [Results] The content of chlorophyll in treatments N, P and K was increased, showing significant difference from that of the control(P<0.05). Among them, Treatment N has the highest content of chlorophyll. Soluble sugar content in treatments N, P and K(K>N>P>CK) was increased considerably and had significant difference from that of the control(P<0.05). Compared with the control, PAL and C_4H activity in treatments N, P and K were increased dramatically by 186.57%, 134.09%, 306.91% and 73.21%, 28.91%, 247.57%, respectively. CHS activity was also increased sharply in treatments N, P and K. Scutellarin, baicalin, wogonoside, baicalein, wogonin and oroxylin A contents in all treatments N, P and K(K>N>P) were increased, and had significant difference from that of the control(P<0.05). [Conclusions] The results showed that macronutrients can increase leaf chlorophyll content, soluble sugar content, PAL activity, C_4H activity and secondary metabolites of S. baicalensis.
[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi. [Methods] The contents of chlorophyll(Chl) and soluble sugar were detected with ultraviolet spectrophotometry. The activities of phenylalanine ammonia-lyase(PAL), cinnamate 4-hydroxylase(C_4H) and chalcone synthase(CHS) were determined with ultraviolet spectrophotometry. Secondary metabolites were detected by high performance liquid chromatography(HPLC). [Results] The content of chlorophyll in treatments N, P and K was increased, showing significant difference from that of the control(P<0.05). Among them, Treatment N has the highest content of chlorophyll. Soluble sugar content in treatments N, P and K(K>N>P>CK) was increased considerably and had significant difference from that of the control(P<0.05). Compared with the control, PAL and C_4H activity in treatments N, P and K were increased dramatically by 186.57%, 134.09%, 306.91% and 73.21%, 28.91%, 247.57%, respectively. CHS activity was also increased sharply in treatments N, P and K. Scutellarin, baicalin, wogonoside, baicalein, wogonin and oroxylin A contents in all treatments N, P and K(K>N>P) were increased, and had significant difference from that of the control(P<0.05). [Conclusions] The results showed that macronutrients can increase leaf chlorophyll content, soluble sugar content, PAL activity, C_4H activity and secondary metabolites of S. baicalensis.
基金
Supported by Special Fund for Public Health Service of Traditional Chinese Medicine Departments([2017]66)
Traditional Chinese Medicine Science and Technology Development Project(2017-522)
