蒺藜TTS-12对须癣毛癣菌的体内外抗菌活性研究

Antibacterial activity of TTS-12 against T. mentagrophytes in vitro and in vivo

为考察天然产物蒺藜TTS-12对须癣毛癣菌的体外和体内抗菌活性,本研究采用CLSI的M38-A2方案对须癣毛癣菌进行最小抑菌和杀菌浓度的检测,选取多重指标考察TTS-12对须癣毛癣菌的抑菌作用;建立豚鼠感染须癣毛癣菌豚鼠体癣模型,分组给药观察低、中、高剂量蒺藜TTS-12凝胶剂对豚鼠体癣模型在背部病变程度评分,病灶皮肤真菌培养阴性率,病灶皮肤病理变化的影响。结果发现,TTS-12对须癣毛癣菌的标株菌株的MIC值为1μg/mL,MFC为8μg/mL,其还能够显著抑制须癣毛癣菌的菌丝生长,浓度为4μg/mL的TTS-12作用15天时,菌丝生长抑制率已经高于60%,且对须癣毛癣菌的孢子萌发抑制作用呈时间和浓度依赖性。动物实验中,低、中、高剂量蒺藜TTS-12凝胶剂均能显著降低背部病变程度评分(P<0.01),中、高剂量能增高病灶皮肤真菌培养阴性率(P<0.05),病灶皮肤HE染色切片表明高剂量组棘层肥厚降低,背部炎症减弱,使角质层恢复正常。以上结果表明蒺藜TTS-12在体外抗须癣毛癣菌和豚鼠体癣模型中均有较好的活性。

In the study,we evaluated the antibacterial activity of TTS-12 against T.mentagrophytes in vitro and in vivo .The M38-A2 method of CLSI was used to detect the minimum inhibitory and fugicide concentration.When guinea pigs were affected,their skin culturing and pathologic changes in local lesions of guinea pig model before and after treatment were record.The effects of low dose,medium dose and high dose of TTS-12 gels on symptom scores were observed.In the treatment period,all treatment group showed a more significant leision score decrease( P <0.01),high dose and medium-dose group increased negative rate on fungal reculture( P <0.05),TTS-12 showed high antibacterial activities against T.mentagrophytes and also had a certain therapeutically effect on guinea pig that infected with Trichophyton mentagrophytes .