裸花紫珠治疗烧烫伤模型大鼠活性部位的筛选及其作用机制研究

Study on the screening and functional mechanism of active parts from Callicarpa nudiflora in the treatment of burns in rats

为筛选裸花紫珠治疗烧烫伤的活性部位,并初步研究其作用机制。实验采用90°C热水造成大鼠烫伤模型。以伤口面积大小,伤口性状,如颜色、软硬程度等为指标,通过宏观和微观检测筛选裸花紫珠治疗烧烫伤的活性部位。采用ELISA和Western blot等方法初步研究其作用机制。进入组织增生重塑期后,石油醚组相较其它各组,伤口愈合最为显著;在组织增生最后的成熟期,石油醚组的表皮和真皮恢复得最好,伤疤最小。石油醚组的VEGF浓度在早期出现了明显的上调;在第21天时,TGF-β1的表达水平最低。裸花紫珠治疗烧烫伤的活性部位初步推测在石油醚层,其治疗烧烫伤作用机制可能是早期裸花紫珠使VEGF上调,在后期下调了TGF-β1以达到。本研究为裸花紫珠治疗烧烫伤的进一步研究和应用提供依据。

To screen the active fractions of Callicarpa nudiflora in the treatment of burns and preliminarily study its action mechanism,so as to provide a basis for future research.The scald model of rats was made by 90 ℃ hot water.With the size of the wound,the characteristics of the wound,such as color,degree of hardness and softness,etc.as the indexes,the active parts of Callicarpa nudiflora treatment of burns were screened by macroscopic and microscopic examination.The mechanism of action was preliminarily studied by ELISA and Western blot.After entering the stage of tissue hyperplasia and remodeling,the wound healing of petroleum ether group was the most remarkable than that of other groups;At the final stage of tissue hyperplasia,the epidermis and dermis of petroleum ether group recovered the best and the scar was the smallest.the VEGF concentration in the petroleum ether group was up-regulated significantly in the early stage;In the petroleum ether group,the concentration of VEGF increased significantly in the early stage,and the expression level of TGF-beta 1 was the lowest on the 21st day.The active parts in the treatment of burns were preliminarily speculated to be in the petroleum ether layer.The mechanism of its effect on burns might be that the early stage of the treatment of burns and scalds by Callicarpa nudiflora up-regulated the expression of VEGF and down-regulated the expression of TGF-beta 1 at the later stage.This study provides proofs for further research and application of Callicarpa nudiflora in the treatment of burns.