牦牛不同组织SREBP-1基因表达差异分析

Differential Analysis of SREBP-1 Gene Expression in Different Tissues of Yak

在不同季节分别采集青海省祁连县、大通县和湟中县牦牛的肝脏、肾脏和结肠,提取Total RNA,在逆转录聚合酶的作用下生成cDNA,利用Real-time PCR检测牦牛甾醇类调控元件结合蛋白1(SREBP-1)基因mRNA的表达水平。结果显示,牦牛不同组织中SREBP-1基因相对表达量由大到小顺序为,肝脏、肾脏、结肠。SREBP-1基因在不同季节中除了2016年春季的牦牛,其他的均以肝脏样品中的SREBP-1 mRNA相对定量拷贝数最高。不同季节间SREBP-1基因在2015年春季和2016年春季中相对表达量差异极显著。3个组织间SREBP-1基因在结肠和肝脏中相对表达量差异呈极显著(P<0.01),在肾脏和肝脏中相对表达量差异显著(P<0.05)。

The liver, kidney and colon of Qiong county, Datong county and Yuzhong county of Qinghai province were collected in different seasons. Total RNA was extracted, cDNA was generated by reverse transcriptase polymerase, and yak sterol regulation was detected by Real-time PCR. Expression level of the element binding protein 1 (SREBP-1) gene mRNA. The results showed that the relative expression levels of SREBP-1 gene in different tissues of yak were from liver to kidney and colon. The SREBP-1 gene has the highest relative copy number of SREBP-1 mRNA in liver samples except for the yak in the spring of 2016. The relative expression of SREBP-1 gene in the spring of 2015 and spring of 2016 was extremely significant. The relative expression of SREBP-1 gene in the colon and liver was significantly (P<0.01), and the relative expression in the kidney and liver was significantly different(P<0.05).