不同中医证型肝硬化患者淋巴细胞亚群及补体系统差异性分析

Analysis of lymphocyte subsets and complement system in patients with liver cirrhosis of different TCM syndromes

目的:分析不同中医证型肝硬化患者淋巴细胞亚群及补体系统差异性。方法:选取2016年2月至2018年2月间在本院接受治疗的肝硬化患者184例,中医证型中64例为脾肾阳虚型、52例为气滞湿阻型、68例为肝肾血淤型。选取同期在本院进行体检的健康者60例作为对照组;流式细胞分析仪检测上述对象NK细胞和T细胞亚群含量,速率散射比浊法检测血清补体B因子、C_3、C_4含量,对检测结果行统计分析。结果:脾肾阳虚、肝脾血淤及气滞湿阻型患者血清NK细胞含量低于对照组,脾肾阳虚型又低于肝脾血淤、气滞湿阻型,脾肾阳虚和肝脾血淤型患者CD4^+、CD3^+、CD4^+/CD8^+比值均低于对照组和气滞湿阻型患者,差异均有统计学意义(P<0.05);脾肾阳虚、肝脾血淤及气滞湿阻型患者血清补体B因子、C_3、C_4含量低于对照组,脾肾阳虚及肝脾血淤型患者血清补体B因子、C_3、C_4含量又低于气滞湿阻型患者,差异均有统计学意义(P<0.05)。结论:肝硬化患者其血清内补体与淋巴细胞亚群含量改变程度不一致,这和患者中医证型有关,这为中医角度分析肝硬化患者的肝细胞受损及免疫状况提供了一些借鉴。

Objective: To analyze the differences of lymphocyte subsets and complement systems in patients with cirrhosis of different syndromes.Methods:A total of 184 patients with liver cirrhosis who were treated at our hospital from February 2016 to February 2018 were selected.Of the TCM syndromes,64 were spleen-kidney yang deficiency,52 were qi-dampity-resistance,and 68 were Liver and kidney blood stasis.Sixty healthy subjects who underwent physical examination in our hospital during the same period were selected as the control group.The levels of NK cells and T cell subsets were measured by flow cytometry,and the levels of serum complement B,C3,and C4 were measured by velocity nephelometry.Statistical analysis of test results.Results: The levels of serum NK cells in patients with spleen and kidney yang deficiency,liver and spleen blood stasis,and qi stagnation and dampness were lower than those in the control group.The spleen and kidney yang deficiency type was lower than that in liver and spleen blood stasis,qi stagnation and wet resistance,and spleen and kidney yang deficiency.The ratios of CD4^+,CD3^+ and CD4^+/CD8^+ in patients with hepatosplenic and blood stasis were lower than those in the control group and qi stagnation and dampness-type patients,with statistically significant differences(P<0.05);spleen-kidney yang,hepatosplenic blood stasis and gas The levels of serum complement B,C3,and C4 in patients with stagnation and dampness were lower than those in the control group.The levels of serum complement B,C3,and C4 in patients with spleen and kidney yang deficiency and hepatosplenic blood stasis were lower than those in patients with qi stagnation and dampness.All had statistical significance(P<0.05).Conclusion: The degree of changes in serum complement and lymphocyte subsets in patients with liver cirrhosis is inconsistent,which is related to the TCM syndrome type of patients.This provides some references for the analysis of liver cell damage and immune status in patients with cirrhosis.