毛细支气管炎患儿血清血管内皮生长因子检测意义

Detection of serum vascular endothelial growth factor in children with bronchiolitis

为探讨血清血管内皮生长因子在毛细支气管炎患儿病程中的变化,收集临床确诊的毛细支气管炎患儿48例,分为重症组(22例)、轻症组(26例),另随机选取20名门诊健康体检儿为健康对照组。各组间年龄、性别、体重无显著性差别。三组人员于入院第1天和第7天采静脉血3 m L,3 000 r/min离心10 min,吸取上清,用ELISA法检测血清血管内皮生长因子浓度,组间t检验分析。血清血管内皮生长因子治疗前重症组明显高于轻症组及健康对照组。重症组治疗前后差异显著(P <0. 01);轻症组治疗前后无显著差异(P> 0. 05)。血管内生长因子在毛细支气管炎重症患儿表达增高,提示可能成为该病严重程度的标志。

To investigate the changes of serum vascular endothelial growth factor in the course of bronchiolitis in children,48 clinically diagnosed children with bronchiolitis were divided into severe group(22 cases)and mild group(26 cases).Another 20 out-patient healthy children were randomly selected as healthy control group.There was no significant difference in age,sex and weight among groups.On the first day and the seventh day of admission,the venous blood of the three groups was collected for 3 mL,centrifuged for 10 minutes at 3 000 r/min,and the supernatant was extracted.The concentration of serum vascular endothelial growth factor was detected by ELISA and analyzed by intergroup t test.Serum vascular endothelial growth factor was significantly higher in severe group than in mild group and healthy control group before treatment.There was significant difference between severe group and mild group before and after treatment(P<0.01),but there was no significant difference of mild group before and after treatment(P>0.05).The increased expression of endovascular growth factor in children with severe bronchiolitis suggests that it may be a marker of the severity of the disease.