摘要
目的阐明一种多肽标签Lucy-tag对于海七鳃鳗(Petromyzon marinus)嗅觉受体膜表达的促进作用。方法用免疫细胞化学、双荧光素酶报告基因(Luciferase)系统和钙流检测法,验证多肽标签Lucy-tag对于海七鳃鳗嗅觉受体的作用。结果多肽标签Lucy-tag促进了20个嗅觉受体中13个嗅觉受体的膜表达,并且Lucy-tag不影响嗅觉受体的固有活性,Lucy-tag也不影响G蛋白偶联受体(G protein coupled receptor,GPCR)的IP_3信号通路。结论该技术为寻找嗅觉受体的配体提供了有利的技术手段,为进一步深入研究GPCR与配体的功能奠定了良好的理论基础。
Aim To elucidate the effect of Lucy-tag polypeptide label on the expression of olfactory receptor membrane in sea lamprey ( Petromyzon marinus ). Methods Immunocytochemistry,double luciferase system and calcium flow detection were used to verify the effect of polypeptide label Lucy-tag on olfactory receptors of sea lamprey. Results Polypeptide label Lucy-tag promoted the membrane expression of 13 olfactory receptors out of 20 olfactory receptors,and Lucy-tag did not affect the intrinsic activity of olfactory receptors,nor did it affect the IP 3 signaling pathway of G protein coupled receptor (GPCR). Conclusions It provides a favorable technique for finding the ligand of olfactory receptor and lays a good theoretical foundation for the further study of the function of the GPCR and ligand.
作者
高翔
张哲
李伟明
任建峰
祖尧
张庆华
GAO Xiang;ZHANG Zhe;LI Wei-ming;REN Jian-feng;Zu Yao;ZHANG Qing-hua(Key Lab of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai201306,China;International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai201306,China;National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai201306,China;Dept of Fisheries and Wildlife,Michigan State University,East Lansing,MI 48824,USA)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第5期727-732,共6页
Chinese Pharmacological Bulletin
基金
中美海洋研究中心基金资助项目(No A1-0209-15-0806)
上海市教委水产高峰学科资助项目
