沙棘CAT家族基因的生物信息学分析

Bioinformatic Analysis of CAT Gene Family in Sea Buckthorn

CAT (Cationic amino acid transporters)是植物体内参与氨基酸的吸收与转运的一类跨膜转运蛋白。为探究CAT家族基因及其所编码蛋白的结构特征,本研究依据前期所获得的不同发育期沙棘果实的转录组测序数据,经过功能注释与分析后获得3个CAT家族成员基因,分别命名为HrCAT1、HrCAT2和HrCAT3,并对其进行生物信息学分析。结果表明,沙棘果实HrCAT家族基因均含有完整的开放阅读框,编码二级结构以α-螺旋为主、不含信号肽的非分泌蛋白,其中HrCAT1和HrCAT2蛋白的三级结构之间具有较高相似性。研究结果可为后续研究沙棘果肉HrCAT基因的表达调控提供理论依据。

Cationic amino acid transporter (CAT) is involved in the uptake and transport of amino acids in plants. The aim of this paper was to study the CAT family genes and structural characteristics of encoded proteins. According to our previous RNA sequencing data from developing fruits of sea buckthorn, the genes encoding for the CAT transport proteins were functionally annotated and a total of three genes were characterized to encode for the CAT family, named as HrCAT 1, HrCAT2 and HrCAT3, respectively. The results of bioinformatics analysis showed that all the identified HrCAT genes contained complete open-reading frame(ORF), which could encode α-helix secondary structure, and non-secretory protein without signal peptide. There was a high similarity in tertiary structure between of HrCAT1 and HrCAT2 proteins. These results could provide a theoretical basis for further studies on the expression regulation of HrCAT genes in sea buckthorn fruit.