摘要
为明确山东泰安本地蓝莓采后果实灰霉病及肉桂精油对其的抑菌效果,本研究从自然发病的蓝莓果实上分离菌株,采用传统真菌形态学、r DNA-ITS序列分析,结合构建系统进化发育树进行鉴定,并通过体外熏蒸抑菌试验研究肉桂精油的抑菌效果。结果表明,该病原菌为灰葡萄孢霉(Botrytis cinerea)。肉桂皮精油对灰葡萄孢霉(Botrytis cinerea)的最低抑菌浓度MIC及最小杀菌浓度MFC分别为0.03μL·mL^(-1)和0.09μL·mL^(-1)。
The study was aimed to identify gray mold rot pathogen in postharvest blueberry, in Taian, Shandong province and the antifungal effect of cinnamon oil. Based on traditional morphological identification method, rDNA-Internal transcribed spacer (ITS) analysis and phylogenetic tree, the strain was identified as Botrytis cinerea. Cinnamon bark oil exhibited obvious antifungal effect on Botrytis cinerea, and the MIC and MFC were 0.03 μL·mL^-1 and 0.06 μL·mL^-1, respectively.
作者
王丹
辛力
张静
杨娟侠
翟浩
董成虎
WANG Dan;XIN Li;ZHANG Jing;YANG Juanxia;ZHAI Hao;DONG Chenghu(Shandong Institute of Pomology,Tai'an,Shandong 271000,China;National Engineering and Technology Research Center for Preservation of Agricultural Products,Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Tianjin 300384,China)
出处
《天津农业科学》
CAS
2019年第5期63-66,共4页
Tianjin Agricultural Sciences
基金
农业部农产品贮藏保鲜实验室开放基金(KF2018006)
山东省果树研究所青年科研基金(2018KY06)
山东省现代农业产业技术体系水果创新团队建设项目(SDAIT-03-022-11)
天津市林果产业技术体系(ITTFPRS2018009)
关键词
蓝莓
灰霉病
肉桂精油
体外熏蒸
blueberry
gray mold rot
cinnamon oil
in vitro fumigation
