马铃薯种质资源组培苗耐盐性评价与耐盐种质筛选

Comprehensive Evaluation of Salt Tolerance and Screening for Salt Tolerant Accessions of Potato Germplasm in vitro

以前期从710份种质中筛选出的29份对盐比较不敏感的马铃薯种质为材料,在含0.75%NaCl的MS培养基上培养20 d后测定组培苗的植株高度、单株根数、单株鲜重和叶片叶绿素含量,以这四个指标的测定值及其耐盐系数为评价依据,结合各指标耐盐系数及测定值隶属函数的聚类分析,对供试种质的耐盐性进行综合评价,筛选出2份耐盐性较强的种质SD400和SD751。在1.00%和1.25%NaCl胁迫下,对这2份高度耐盐种质的耐盐性进行进一步鉴定,结果表明,对1.00%NaCl胁迫仍有一定的适应性,SD400和SD751叶绿素含量分别为对照的27.52%和53.59%,株高分别为对照的21.92%和29.05%,单株根数分别为对照的26.61%和45.07%;但在1.25%NaCl胁迫下仅SD751有微量生长。

Using 29 salt-insensitive accessions earlier screened from 710 potato germplasm resources as materials, their plant hight, root number and fresh weight per plant and chlorophyll content were measured after cultured in MS containing 0.75% NaCl for 20 days. Based on these four indexes and their salt tolerance coefficients, the salt tolerance of the tested accessions were comprehensively evaluated by the clustering analysis of the membership function of the index value and their salt tolerance coefficients. Two highly salt-tolerant accessions, SD400 and SD751, were screened out. Their salt tolerance was identified under 1.00% and 1.25% NaCl, and the results showed that they had strong adaptability to 1.00% NaCl stress. Under 1.00% NaCl stress, the chlorophyll content of SD400 and SD751 were 27.52% and 53.59% of that of control, the plant height was 21.92% and 29.05% of that of control,and the root number per plant was 26.21% and 45.07 of that of control, respectively. SD751 also grew in a micro amount under 1.25% NaCl stress.