摘要
构建了以卟啉多孔化合物-金纳米颗粒(Au NPs@PAF)为固定基质的无标记型C-反应蛋白(CRP)光电免疫传感器。在最优条件下,采用计时电流法实现了对CRP的定量检测。该传感器在CRP质量浓度0.05~60 ng/mL范围内与光电流有较好的线性关系,检出限为0.017 ng/mL,相关系数为0.994 6,平均回收率为102%,具有良好的选择性,为C-反应蛋白的检测提供了一种灵敏的方法。
In this paper,a label-free C-reactive protein(CRP)photoelectricimmunosensor was constructed using porous porphyrin compound-Au nanoparticles(Au NPs@PAF)as an immobilized substrate.Under the optimal conditions,the CRP was quantitatively detected by chronograph current method.There was a good linear relationship for the sensor versus photocurrent in the range of 0.05-60 ng/mL,with a linear correlation coefficient of 0.994 6.The limit of detection was 0.017 ng/mL,and the average recovery was 102%.This sensor provided a sensitive method for the detection of C-reactive protein with good selectivity.
作者
王敏
孟爽
谷梦巧
迟宽能
马玉婵
邓燕
李德蕾
张茜
胡蓉
杨云慧
WANG Min;MENG Shuang;GU Meng-qiao;CHI Kuan-neng;MA Yu-chan;DENG Yan;LI De-lei;ZHANG Xi;HU Rong;YANG Yun-hui(College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2019年第3期283-288,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(21465026
21765026)
关键词
卟啉多孔材料
光电免疫传感器
C-反应蛋白
应用
porphyrin porous materials
photoelectric immunosensor
C-reactive protein
application