摘要
In order to improve the degradation activity of organophosphorus degradation bacterial strains, uniform design and support vector regression(SVR) are used to optimize the formula of mineral salt medium for the strains based on single factor experiment. The formula being used after SVR optimization is glucose 1.24 g/L,NH4 NO3 0.73 g, KH2 PO4 0.96 g, KCl 0.50 g, MgSO4·7 H2 O 0.50 g, MnSO4 0.02 g, CaCl2 0.04 g, distilled water 1 000 mL;the highest degradation rate is obtained at pH7.0(SVR, 76.68%), superior to that of the QSAR model(74.67%). The formula optimization method based on uniform design and SVR is very promising to apply in various multiple-factor and multiple-level formula optimization experiments.
为提高有机磷降解菌菌株的降解活性,在单因素实验的基础上采用均匀设计和支持向量回归(SVR)对菌株的无机盐培养基配方进行优化。结果表明,当经SVR优化后的培养基配方为葡萄糖1.24 g/L、NH_4NO_30.73 g、KH_2PO_4 0.96 g、KCl 0.50 g、Mg SO_4·7H_2O0.50 g、MnSO_4 0.02 g、CaCl_2 0.04 g、蒸馏水1 000 mL、pH 7.0时,降解率最高(SVR,76.68%),优于二次多项式逐步回归分析参比模型(QSAR,74.67%)。基于均匀设计和SVR的配方优化方法有望在多因素多水平配方优化试验中得到广泛应用。
基金
Supported by Innovation Platform Project of Colleges and Universities of Hunan Province(14K053)
Youth Fund of Hunan Institute of Humanities,Science and Technology(2015QN09)~~