棉籽蛋白脱酚方法研究

Removal of Gossypol from Cottonseed Protein

以棉籽粕为原料,采用碱提酸沉法提取棉籽蛋白,对酸沉得到的蛋白质用蒸馏水、乙醇、双氧水、亚硫酸氢钠进行洗涤以进一步去除棉酚,经过对比选择亚硫酸氢钠作为脱除剂,并对其脱除条件进行优化。得到亚硫酸氢钠脱除棉酚的最佳条件为添加量为0.3%、温度为40℃,搅拌时间为2h。在最佳条件下,棉酚脱除率达到了68.92%,蛋白质含量为85.47%。

Using cottonseed meal as raw material,this study extracted cottonseed protein by alkali extraction and acid precipitation.With distilled water,ethanol,hydrogen peroxide and sodium sulfite,the protein obtained from acid precipitation was washed to further remove gossypol.Sulfurous acid was selected through comparison as a remover and its removal conditions were then optimized.The optimum conditions for obtaining gossypol to remove gossypol are the added amount at 0.3%,the temperature at 40℃,and the stirring time with 2 h.Under the optimal conditions,the gossypol removal rate reached 68.92%and the protein content was 85.47%.