玉米JAZ家族基因的表达规律分析

Expression pattern of JAZ family genes in Zea mays

为阐明玉米JAZ家族基因的功能及其调控机制,本研究利用生物信息学方法,对玉米JAZ家族基因进行系统进化分析、保守结构域分析、组织特异性分析以及在玉米抵抗生物和非生物胁迫过程中的表达规律分析。利用Real-time PCR技术,检测玉米JAZ家族基因在玉米抗感禾谷镰孢的自交系Mo17和B73中的表达规律。结果发现,玉米JAZ家族基因与拟南芥家族基因具有一定同源性,23个成员都含有TIFY和Jas(CCT_2)结构域且具有组织特异性;在生物(黄曲霉和拟轮枝镰孢)和非生物胁迫(高温、低温、高盐和紫外损伤)下,表达水平呈现明显的变化;部分JAZ家族基因在玉米抗感禾谷镰孢自交系Mo17和B73中的表达规律呈现明显的差别,说明JAZ家族基因在玉米抵抗生物和非生物胁迫以及玉米抵抗禾谷镰孢侵染过程中发挥重要的作用。

To make clear of the function and regulation mechanism of JAZ family genes in Zea mays, bioinformatics method was used in this study for phylogenetic analysis, conserved domain analysis, tissue-specificity analysis, and expression pattern analysis of maize in resistance to biotic and abiotic stress. Real-time PCR was used to detect the expression of maize JAZ family genes in maize inbred lines Mo17(resistant to Fusarium graminearum) and B73(susceptible to Fusarium graminearum).The results showed that the maize JAZ family genes were homologous with the Arabidopsis family genes, and all the 23 JAZ family members had the domain structure of TIFY and Jas(CCT2), expressing tissue-specificity. The expression level showed obvious changes under the biological stress(Aspergillus flavus and Fusarium verticillioides) and abiotic stress(high temperature, low temperature,high salt and ultraviolet damage). The expression patterns of some JAZ family genes were significantly different in inbred lines Mo17 and B73. The results indicate that the JAZ family genes play an important role in the resistance to biotic and abiotic stresses in maize as well as an important role in the fight against Fusarium graminearum infection.