白刺花胚性愈伤组织诱导及体细胞胚发生和萌发

Embryonic Callus Induction of Sophora davidii and Their Somatic Embryogenesis and Germination

探讨不同因素对白刺花下胚轴、子叶2种外植体胚性愈伤组织诱导及体细胞胚发生和萌发的影响。以B5和MS为基本培养基,研究2,4-D、6-BA和TDZ对白刺花下胚轴和子叶胚性愈伤组织的诱导;在MS培养基上添加不同浓度2,4-D,研究胚性愈伤组织增殖情况;采用ABA,探究对体细胞胚发生的影响。结果表明:下胚轴比子叶更易诱导胚性愈伤组织,筛选出2种外植最佳的胚性愈伤组织诱导培养基均为MS+2.0 mg/L 2,4-D+0.5 mg/L TDZ+0.5 mg/L 6-BA,胚性愈伤组织诱导率分别为77.3%和41.0%。15.0 mg/L ABA、0.2 mg/L 2,4-D和2.0 mg/L 6-BA有利于体细胞胚发生,1/3MS+0.2 mg/L NAA+0.1 mg/L 6-BA+2.0 g/L活性炭+25 g/L蔗糖+7 g/L琼脂的培养基可使体细胞胚萌发率达80%以上,再生植株移栽成活率高达90%。白刺花外植体种类及培养基类型均会影响胚性愈伤组织的诱导,其中下胚轴诱导效果优于子叶;MS培养基较适合启动细胞脱分化形成愈伤组织,2,4-D对胚性愈伤组织的增殖保持有调控作用,ABA有利于体细胞胚的发生。

This work is to investigate the effects of different factors on embryonic callus induction,somatic embryogenesis and somatic embryo germination from the hypocotyl and cotyledon of Sophora davidii.Having B5 and MS as the basic medium,the inductions of 2,4-D,6-BA and TDZ on embryonic callus from the hypocotyl and cotyledon of S.davidii,the proliferation of the embryonic callus in the MS medium supplied with different concentration of 2,4-D,and the effects of ABA on somatic embryogenesis were studied.The results showed that the embryonic callus induction from the hypocotyl more easily occurred than from cotyledon.The optimal medium for the embryo callus induction for two explants were MS+2.0 mg/L 2,4-D+0.5 mg/L TDZ+0.5 mg/L 6-BA,the induction rate was 77.3%and 41.0%respectively.The 15 mg/L ABA+0.2 mg/L 2,4-D+2.0 mg/L 6-BA was conducive to the somatic embryogenesis.The somatic embryo germination rate was up to 80%in the medium of 1/3 MS+0.2 mg/L NAA+0.1 mg/L 6-BA+2.0 g/L activated carbon+25 g/L sucrose+7g/L agar.The survival rate of transplanted seedlings reached 90%.In sum,the types of the explants and medium may affect the induction of embryonic callus,for which hypocotyl was better than cotyledon.MS medium is more suitable for initiating cell dedifferentiation to generate callus.2,4-D may regulate the proliferation and maintenance of embryonic callus,and ABA is favorable for somatic embryogenesis.