分子标记辅助选择不同甜瓜性别植株

Molecular Marker-assisted Selection in Melon Sex Expression

运用A和G基因鉴定甜瓜的性别类型,为甜瓜其他性状的分子标记辅助育种提供方法。根据已发表的甜瓜CmACS-7(A基因)与WIP1+Gyno-hAT(G基因)基因结构设计PCR引物,对47个重组自交系群体家系及14份甜瓜材料进行检测验证。引物CmACS-7扩增产物在383 bp进行酶切,基因型为AA不能被酶切,扩增产物均被酶切的为基因型aa,酶切位点可分辨共显性基因型;针对WIP1+Gyno-hAT基因结构设计2对引物同时扩增,扩增条带197 bp和184 bp区别G(g)基因基因型。利用自主设计的引物可用于甜瓜苗期筛选雌雄异花同株、全雌系、雄全同株和完全花植株的分子标记辅助选择育种。

The objective of this study is to identify the sex type of melon via using A and G genes,and to provide a method for molecular marker-assisted breeding of other traits in melon.Based on the structures of the published CmACS-7(A)gene and WIP1+Gyno-hAT(G)gene in melon,PCR primers were designed,by which the detection of 47 recombinant inbred lines families and 14 melon materials was verified.Results indicated that PCR products from primer CmACS-7 was enzymatically digested at 383 bp,those with the genotype AA was not able to be digested by enzyme,and those digested by enzyme were in genotype aa,moreover,endonuclease locus discernible co-dominant genotype.Two primers were designed based on WIP1+Gyno-hAT gene structure,G(g)genotype was distinguished between 197 and 184 bp of the amplified bands.In conclusion,the self-designed primers can be used in the molecular marker-assisted selection breeding of monoecious,gynoecious,pure male line,and complete-flower melon at seedling stage.