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分光光度计鉴别结核与非结核分枝杆菌的研究 被引量:3

Study on Discrimination of Mycobacterium Tuberculosis From Nontuberculous Mycobacteria Using Spectrophotometer
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摘要 目的建立并评估应用分光光度计测定对氨基苯甲酸(PABA的浓度,快速鉴别结核分枝杆菌(MTB)与非结核分枝杆菌(NTM)的方法。方法首先建立应用分光光度计测定PABA浓度的方法,然后以传统含对硝基苯甲酸(PNB)罗氏培养基鉴定结果为参照,应用32株MTB临床分离株和33株NTM临床分离株评价该方法的准确性。结果以PABA浓度0μg/mL定为cut-off值,还原PNB产生PABA的浓度大于0μg/mL判定为NTM,小于0μg/mL判定为MTB,应用分光光度计测定PABA浓度法鉴定32株MTB临床分离株和33株NTM临床分离株的结果与传统鉴定法完全一致,其灵敏性和特异性均为100%。结论本研究建立了应用分光光度计测定PABA浓度快速鉴别MTB与NTM的方法,该方法简便、快速、廉价,并具有高度的特异性和敏感性,可作为鉴别MTB与NTM的有价值的检测手段。
作者 魏国梅 王桂荣 姜广路 于霞 陈素婷 黄海荣 WEI Guomei;WANG Guirong;JIANG Guanglu;YU Xia;CHEN Suting;HUANG Hairong
出处 《继续医学教育》 2019年第4期133-135,共3页 Continuing Medical Education
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