油樟组织培养技术研究

A Study of Tissue Culture of Cinnamomum longepaniculatum

以当年生油樟枝条为研究对象,探索了消毒剂种类、浓度及其作用时间、MS培养基、6-BA、NAA、IBA等5种因素对油樟茎段组织培养的影响,分析筛选出了一套能够产出具有优良素质油樟种苗的组织培养技术。结果表明:油樟茎段经10%84处理15min或15%84处理10 min两种方法进行消毒均能获得污染率<15%,且成活率>70%的无菌外植体;适合不定芽诱导的培养基为:蔗糖30 g·L^(-1)+卡拉胶6 g·L^(-1)+MS+6-BA 1.0 mg·L^(-1)+IBA 0.1 mg·L^(-1);适合不定芽增殖继代的培养基:蔗糖30 g·L^(-1)+卡拉胶6 g·L^(-1)+MS+6-BA 1.0 mg·L^(-1)+NAA 0.10 mg·L^(-1)+IBA 0.2 mg·L^(-1);生根培养基:蔗糖15 g·L^(-1)+卡拉胶6 g·L^(-1)+1/2MS+IBA 1.5 mg·L^(-1)+NAA 1.0 mg·L^(-1)。

The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15 min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+ IBA 0.1 mg·L^-1;The medium for multiplication of adventitious buds was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+NAA 0.10 mg·L^-1+IBA 0.2 mg·L^-1;The rooting medium was sucrose 15 g·L^-1+carrageenan 6 g·L^-1+1/2 MS+IBA 1.5 mg·L^-1+NAA 1.0 mg·L^-1.