硒与奶牛免疫能力的相关性研究

Correlation between Selenium and Immunity in Dairy Cows

为分析硒与奶牛免疫能力的关系,试验随机选取年龄、胎次、体况相近的泌乳奶牛50头。根据血清硒水平高低分为对照组和试验组,通过双抗体夹心法检测每组实验动物血液中的IL-2、IL-6、TNF-α含量。结果显示,试验组奶牛血清中的IL-2均低于对照组,但差异不显著(P>0.05),奶牛血清中硒水平与IL-2呈正相关(R=+0.133,P=0.356);试验组奶牛血清中的IL-6均高于对照组,但差异不显著(P>0.05),奶牛血清中硒水平与IL-6呈显著负相关(R=-0.28,P=0.049);试验组奶牛血清中的TNF-α均低于对照组,差异显著(P<0.05),奶牛血清硒水平与TNF-α呈显著正相关(R=+0.311,P=0.028)。该试验表明适当补充硒,可以提升奶牛机体免疫能力。

In order to analyze the relationship between selenium and immune ability in dairy cows,a total of 50 lactating cows with the similar age,parity and body condition were randomly selected and divided into control group and experimental group according to the serum selenium level.The contents of IL-2,IL-6 and TNF-α in the blood samples of dairy cows were determined by double antibody sandwich method.The results showed that while the serum content of IL-2 of experimental group was lower than control group,no significant difference was observed (P>0.05);there was a positive correlation between serum selenium level and serum content of IL-2 (R=+0.133,P=0.356);while the serum content of IL-6 of experimental group wase higher than control group,no significant difference was observed (P>0.05);there was a significantly negative correlation between serum selenium level and serum content of IL-6 (R=-0.28,P=0.049);the serum content of TNF-α of experimental group was significantly lower than control group (P<0.05);there was a significantly positive correlation between serum selenium level and serum content of TNF-α(R=+0.311,P=0.028).The combined data suggest that the selenium supplementation with appropriate level can improve the immune ability of dairy cows.