七氟烷对新生大鼠海马caspase-3和磷酸化ERK表达的影响

Effects of sevoflurane anesthesia on expression of caspase-3 and phosphorylated ERK in neonatal rat hippocampus

目的评价磷酸化细胞外信号调节激酶(p-ERK)在七氟烷致新生大鼠海马神经细胞凋亡中的作用。方法出生7 d的健康清洁级雄性SD大鼠36只,体质量11～18 g,采用随机数字表法分为对照组(C组,n=18)和七氟烷麻醉组(S组,n=18)。C组每天同一时间吸入2 h的30%氧气;S组吸入2 h的3.4%七氟烷。2 d后处死大鼠采用Western blotting法检测活化caspase-3和p-ERK的表达。结果与C组比较,S组活化caspase-3的表达上调,p-ERK的表达下调(P<0.05)。结论七氟烷通过下调p-ERK的表达促进新生大鼠海马神经细胞凋亡。

Objective To evaluate the role of ERK signaling pathway in sevoflurane anesthesia caused apoptosis in hippocampal neurons in neonatal rat. Methods Thirty two pathogen free healthy male Sprague Dawley 36 rats,aged 7days,weighing 11 18 g,were divided into 2 groups using a random number table:control group (group C) and sevoflurane anesthesia group(group S).In group S, the rats were exposed to 3.4% sevoflurane for 2h,while in group C the rats were exposed to 30% oxygen for 2h at the same time each day .After 2days four rats in each group were sacrificed and hippocampus were removed for determination of activated caspase 3 expression and p ERK by Western blot. Results The level of activated caspase 3 in hippocampus was significantly higher in group S than in group C ( P <0.05).Compared with group C,the expression of p ERK was down regulated in group S ( P <0.05). Conclusion Sevoflurane anesthesia causes the neuronal apoptosis in neonatal rat hippocampus through down regulating the expression of p ERK in hippocampus.