交流电场对铀胁迫下黑麦草种子萌发的影响

Effect of alternating current electric field on germination of ryegrass seed under uranium stress

通过研究促进植物种子在铀胁迫条件萌发和生长的方法,对于开展铀污染场地植物修复和防护具有重要意义。研究主要探讨了黑麦草(Lolium perenne L.)种子经过不同条件交流电场处理后,在铀胁迫下的萌发及生长变化。结果表明,1 mmol/L铀处理对黑麦草种子发芽相关指标及幼苗生长均造成了显著的胁迫作用(P<0.05),且施加不同条件的交流电场对黑麦草种子在铀胁迫下的发芽率和发芽指数影响较小,对发芽势和活力指数影响较大。此外,在高、中、低3个电场处理条件下(1.5、15.0、150.0 V),2 h的各电场处理会加剧铀胁迫对黑麦草幼苗株高、根长和生物量的影响;时长较长时,除了在24 h高电场处理也是促进胁迫外,12、24 h各强度电场处理却能缓解铀胁迫对黑麦草幼苗株高、根长和生物量的影响。表明采取适宜的电场处理强度和处理时间策略可能对铀胁迫下植物种子的萌发指标影响不大,但是能够增强植物幼苗的抗逆性。

It is of great significance to develop phytoremediation by studying methods for promoting seed germination and plant growth of plant under uranium stress. The effect of different treatments of alternating current(AC) electric field on ryegrass(Lolium perenne L.) seed germination and seedling growth under uranium stress was studied. Results showed that, the treatment of uranium(1 mmol/L) caused significant stress on ryegrass in the period of seed germination and seedling growth(P<0.05), and the treatments with electric field had less influence on the germination rate and germination index, but had larger influence on germination potential and vigor index of ryegrass seed under uranium stress. Besides, at high, medium and low level of electric field(1.5 V,15.0 V,150.0 V), the treatments with electric field in 2 h showed more serious uranium stress effects on plant height, root length and biomass of ryegrass seedling, however, except the high level of electric field in 24 h, the treatments with electric field in 12 h and 24 h could significantly relieve the effects of uranium stress. With the appropriate electric field strength and treat-time, there may have little effect on the seed germination indicators of ryegrass seed under uranium stress, but it can promote the uranium resistance of seedlings.