酸性染料比色法测定功劳木总生物碱的含量

Determination of the Total Alkaloids of Caulis Mahonia by Acid Dye Colorimetry

目的:采用酸性染料比色法测定功劳木中总生物碱的含量。方法:生物碱与酸性染料结合,生成有色离子对,用有机溶剂萃取,测定有机相中有色离子对特征波长处的吸光度,从而可计算其总生物碱含量。结果:显色后在415 nm处有最大吸收,盐酸小檗碱在20.4～61.2μg/mL范围内成线性关系,平均回收率为97.96%,RSD为4.1%(n=5)。结论:该方法灵敏度高、重复性好。

Subjective: Content quality evaluation of Caulis Mahonia and its total alkaloids. Method: Acid dye colorimetry, In pH=5.2 conditions, the alkaloids combined with acid dyes, which generated the ion absorption Spectra clearly redshift, the ion-pair can be quantified by organic solvent extraction, determination of ferrous ion in organic solvents on the characteristic wavelength of absorbance, which then turn off the determination of content of Total alkaloids from Caulis Mahonia, and recovery experiments. Results: The maximum absorption is 415 nm, Berberine hydrochloride as in 20.4~61.2 μg/mL range linear relationship, the average recovery was 97.96 %, RSD was 4.1 %(n=5). Conclusion: The method uses less high sensitivity and good reproducibility.