产朊假丝酵母、植物乳杆菌混合发酵及净水效果

Mixed fermentation of Candida utilis and Lactobacillus plantarum and its water purification effect

采用单因素试验和正交试验的方法对产朊假丝酵母Candida utilis和植物乳杆菌Lactobacillus plantarum组成的水产微生态制剂EM12的混合发酵技术及发酵产物的水质净化效果进行了研究。EM12在优化的条件下混合发酵,植物乳杆菌和产朊假丝酵母液体发酵的最高活菌浓度分别达6. 70×109、2. 00×107CFU/m L,固体发酵的最高活菌浓度分别达1. 64×1010、1.65×108CFU/g湿培养基。固体和液体菌剂对模拟水体中45. 0 mg/L亚硝态氮去除率分别为100%和89. 8%。产朊假丝酵母和植物乳杆菌混合同步发酵,提高了有效活菌浓度,保证了水质净化效果,为该复合菌剂在水产养殖上的应用提供了理论基础和技术支撑。

A microbiological agent named EM12 was consisted of Candida utilis and Lactobacillus plantarum.Single factor experiments and orthogonal-design experiments were applied to optimize its liquid-and solid-state mixed fermentation technology,followed by examining the water purification efficiencies of the fermented products. The results showed that under the optimized liquid-state medium and fermentation conditions,the densities of L. plantarum and C. utilis reached up to 6. 70 × 10^9 CFU/m L and 2. 00 × 10^7 CFU/m L,respectively. Moreover,with the optimal solid-state medium,the densities of L. plantarum and C. utilis reached up to 1. 64 × 10^10 CFU/g and 1. 65 × 10^8 CFU/g,respectively. The efficiencies of removing 45. 0 mg/L nitrite from simulated sewage by solid and liquid microbial inoculums were 100% and 89. 8%,respectively. Mixed fermentation of L. plantarum and C. utilis not only increased the concentration of effective live bacteria,but also ensured their water purification effects,which provides theoretical basis and support for applying these compounded microbial inoculums in aquaculture.