石斛灵芝口服液增强小鼠免疫功能的研究

Dendrobium and Ganoderma lucidum oral liquid on immunity enhancement of mice

目的评价石斛灵芝口服液对昆明种雌鼠免疫功能的影响。方法选用正常昆明种雌鼠160只,分为3.33、6.67、10 m L/kgbw石斛灵芝口服液3个剂量组和阴性对照组,连续灌胃30 d后,取胸腺和脾称重并计算脏体比值,测定体液免疫功能(抗体积数和溶血空斑数)、细胞免疫功能(光密度值和足跖肿胀度)、单核-巨噬细胞吞噬功能(吞噬指数和吞噬率)和NK细胞活性,其中2项出现阳性结果判定石斛灵芝口服液具有增强免疫功能的作用。结果 4组雌鼠的脏体比值、抗体积数、光密度差值、吞噬指数、吞噬率和NK细胞活性比较,差异均无统计学意义(P>0.05);中、高剂量组雌鼠的溶血空斑数分别为(315.5±49.9)/106脾细胞和(343.0±43.8)/106脾细胞,均多于对照组的(249.5±41.3)/106脾细胞(P<0.05);中、高剂量组雌鼠的足跖肿胀度分别为(1.34±0.42)、(1.37±0.49) mm,均高于对照组的(0.89±0.37) mm (P<0.05)。结论石斛灵芝口服液具有增强昆明种雌鼠免疫功能的作用。

Objective To evaluate the immune function of Dendrobium and Ganoderma lucidum oral liquid in female KM mice.Methods A total of 160 healthy female KM mice were selected and divide into three dose groups and a control group.Dendrobium and Ganoderma lucidum oral liquid was administered to the mice in the three dose groups at 3.33,6.67 and 10.00 mL/kgbw by intragastric lavage for 30 days,while distilled water was given to the mice in the control group in the same way.The thymus and spleen were weighed and the visceral organ coefficients were calculated.The humoral immune function,cellular immune function,monocyte-macrophage phagocytosis function and natural killer(NK)cell activity were detected,then the effects of the oral liquid on immunity enhancement of mice could be proved with any two items above positive.Results No statistically significant differences were found between the dose groups and the control group in the visceral organ coefficients,serum hemolysis,lymphocyte proliferation ability,macrophage phagocytosis and NK cell activity(P>0.05).In the middle- and high-dose group,the number of plague-forming cells per 106 spleen cells were 315.5±49.9 and 343.0±43.8,respectively,which were more than that in the control group(249.5±41.3,P<0.05);the capacity of toe swelling in the middle- and high-dose group were(1.34±0.42)and(1.37±0.49)mm,respectively,which were more than that in the control group [(0.89±0.37)mm,P<0.05].Conclusion Dendrobium and Ganoderma lucidum oral liquid have the effect of enhancing immunity on female KM mice.