基于脂质体反应器的酶纳米生物传感器在敌敌畏快速检测中的应用

Fabrication of Enzyme Nano-Biosensor Based on Liposome Bioreactor for Rapid Detection of Dichlorvos

采用脂质体技术制备乙酰胆碱酯酶的微反应器,并以微反应器、壳聚糖和二氧化硅为基质,构建以多层纳米酶膜修饰电极为核心的有机磷农药残留快速检测酶电化学生物传感器。结果表明,酶脂质体微反应器平均粒径为(7.26±0.75)μm;修饰电极多层酶膜的最佳层数为6个双层;以敌敌畏作为有机磷农药模型,分别在其浓度为0.25～1.75μmol/L和2.00～10.00μmol/L线性范围内,传感器的峰电流值与乙酰胆碱酯酶抑制率呈现出良好的线性关系,回归方程分别为I=28.58C+5.35和I=2.38C+51.25;最低检出限为(0.72±0.065)μg/L(信噪比为3);此新型酶纳米生物传感器具有良好的灵敏度、重复性和选择性。

Acetylcholinesterase (AChE) liposomes bioreactor (ALB), prepared by encapsulating the enzyme AChE into L-α-phosphatidylcholine liposomes, was spherical in shape. A novel AChE biosensor based on multilayer films containing SiO 2 , chitosan (CS) and ALB was developed. The results showed that ALB had a mean diameter of (7.26 ± 0.75)?m. The biosensor based on six bilayers of multilayer films was the best. The properties of the biosensor were evaluated by electrochemical measurements. Based on the inhibition of organophosphate pesticides on the AChE activity, by using dichlorvos as a model organophosphorus compound, we observed that the inhibition of dichlorvos was proportional to its concentration within the range from 0.25 to 1.75 μmol/L and from 2.00 to 10.00 μmol/L, and the corresponding equations were I = 28.58C + 5.35 and I = 2.38C + 51.25, with a detection limit (LOD) of (0.72 ± 0.065)μg/L estimated at a signal-to-noise ratio of 3. The fabrication of the biosensor was simple, with fast response and acceptable selectivity.