绵马贯众多酚的分离纯化及其抗氧化活性研究

Purification and Antioxidation Activity of Polyphenol from Cyrtomium fortune

采用70%乙醇提取绵马贯众多酚,并通过吸附实验考察了7种大孔树脂对绵马贯众多酚粗提物吸附率和解吸率的影响,发现HPD100树脂对绵马贯众多酚的纯化效果较好;进一步分析了样品质量浓度、上样速度、解吸乙醇体积分数、洗脱速度及样品pH值对HPD100树脂纯化绵马贯众多酚的影响,得出最佳纯化工艺条件为:样品溶液质量浓度为2.00 g/L,pH值为5,上样速度为1 mL/min,解吸乙醇体积分数为70%及洗脱速度为2 mL/min。该工艺条件下,绵马贯众多酚纯度由纯化前的27.64%提高到纯化后的54.00%(得率为6.46%),纯化效果显著。纯化后绵马贯众多酚抗氧化活性显著增加,对DPPH自由基(DPPH·)及羟基自由基(·OH)的IC_(50)值分别为0.01和0.49 g/L(纯化前为0.05和1.79 g/L),0.04 g/L时对DPPH·的清除率为90.82%,与Vc相当;质量浓度为0.05 g/L时,纯化后绵马贯众多酚对铁离子还原能力为0.47,明显高于纯化前的0.25;且在酸性条件下DPPH·清除能力以及铁离子还原能力更高,在碱性条件下·OH清除能力更高。

The polyphenols were extracted from Cyrtomium fortune with 70% ethanol, and then purified with 7 macroporous resins. The results showed that HPD100 resin had the best purification effect based on the adsorption rate and desorption rate. The effect of sample mass concentration, loading speed of sample,volume fraction of desorption ethanol, desorption speed and sample pH value on the purification of HPD100 resin was further analyzed, and the optimum purification conditions were obtained as follows:mass concentration of sample solution 2.00 g/L, pH value 5, loading speed of sample 1 mL/min,volume fraction of desorption ethanol 70% and desorption speed 2 mL/min. Under these conditions, the content of polyphenols increased from 27.64% to 54.00%(the yield of polyphenols was 6.46%). The purified polyphenols showed higher antioxidant activity. For the purified polyphenols, the IC 50 of DPPH radical (DPPH ·) and hydroxyl radical (· OH) were 0.01 and 0.49 g/L, respectively;but for the crude polyphenols, the IC 50 of DPPH · and · OH were 0.05 and 1.79 g/L, respectively. At 0.04 g/L, the DPPH · scavenging activity of the purified polyphenols was 90.82%, almost similar to that of Vc. At 0.05 g/L , ferric ion reducing power of the purified polyphenols was 0.47, but the crude polyphenols was only 0.25. Furthermore, the purified polyphenols exhibited better DPPH · scavenging activity and ferric ion reduction ability under acidic conditions and the hydroxyl radical scavenging activity under alkaline condition.