摘要
本研究利用筛选出的10对多态性高、条带清晰的SSR引物,以海南岛68份红毛丹种质为材料,进行遗传多样性分析和SSR指纹图谱的构建。结果表明:10对引物在供试材料中共检测出20个等位位点,多态性条带比例为100%,平均多态性信息含量(PIC)为0.393;对68份红毛丹种质做聚类分析,遗传相似系数为0.290～0.664;采用引物?带型组合法构建了68份红毛丹种质的指纹图谱。该结果为今后红毛丹种质鉴定和分子育种提供重要依据。
The SSR fingerprint map and genetic diversity of 68 rambutan (Nephelium lappaceum L.) accessions were studied using 10 pairs of selected polymorphic SSR primers. The results indicated that all the primers showed polymorphism, and 20 polymorphic alleles were revealed and the average polymorphic information content (PIC) was 0.393. The genetic similarities among accessions ranged from 0.290 to 0.664, on which a phylogenetic tree showing the relationship among the accessions was constructed. A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 68 cultivars. The DNA fingerprinting provides a technical reference for the varieties identification and molecular breeding in rambutan.
作者
林兴娥
牛俊海
陈莹
明建鸿
高宏茂
葛宇
周兆禧
LIN Xing’e;NIU Junhai;CHEN Ying;MING Jianhong;GAO Hongmao;GE Yu;ZHOU Zhaoxi(Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 570102,China;TropicalCrops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China;Instituteof Tropical Agriculture and Forestry,Hainan University,Danzhou,Hainan 571737,China)
出处
《热带作物学报》
CSCD
北大核心
2019年第4期708-714,共7页
Chinese Journal of Tropical Crops
基金
中国热带农业科学院基本科研业务费项目(No.1630092018004
No.1630092017010)
