ZNF207在肝细胞癌中的表达及意义

Expression of ZNF207 in hepatocellular carcinoma and its significance

目的:研究锌指蛋白207(zinc finger protein 207,ZNF207)在人肝细胞癌(hepatocellular carcinoma,HCC)中的表达情况,分析其与HCC临床病理特征及预后的相关性。方法:选择10对新鲜的冰冻HCC组织及相应癌旁肝组织标本,采取real-time PCR检测其中ZNF207 mRNA的表达;另随机选择135例有完整资料的HCC石蜡标本,采用免疫组织化学检测其中ZNF207蛋白的表达;统计分析ZNF207的表达与HCC临床病理特征及预后的关系。结果:在新鲜冰冻HCC组织中ZNF207 mRNA的表达水平明显高于相应的癌旁肝组织(P<0.01);免疫组织化学结果显示HCC组织中ZNF207呈显著高表达;且ZNF207的表达与肝硬化、结节数目、肿瘤包膜、微血管侵犯以及TNM分期明显相关(P<0.05);生存分析显示ZNF207高表达组HCC患者总生存率、无瘤生存率较ZNF207低表达组明显下降(P<0.01);单多因素回归分析显示ZNF207是HCC患者总生存率及无瘤生存率的独立危险因素(P<0.05)。结论:ZNF207在HCC中呈高表达,与HCC不良临床病理特征密切相关,并预示不良预后。

Objective: To investigate the expression of zinc finger protein 207 (ZNF207) in hepatocellular carcinoma (HCC) tissues, and analyze the correlation of ZNF207 with clinicopathological factors and HCC patients’ survival. Methods: Real-time PCR was used to detect ZNF207 mRNA expression in 10 paired fresh HCC and adjacent non-tumor liver tissue samples. Immunohistochemical (IHC) analysis was used to detect ZNF207 protein expression in 135 cases of randomly selected paraffin-embedded HCC tissues. Th e correlation of ZNF207 expression with clinicopathological factors and survival of HCC was analyzed. Results: The ZNF207 mRNA expression level in HCC was significantly higher than that in the adjacent non-tumor liver tissue (P<0.01). IHC results showed that ZNF207 protein level was elevated in HCC tissues and ZNF207 expression was correlated with cirrhosis, nodule number, tumor capsule, vascular invasion, and TNM stage (P<0.05). Survival analysis showed that patients with high ZNF207 expression had poorer overall survival (OS) and disease-free survival (DFS) than those with the low ZNF207 expression (P<0.01), and ZNF207 was an independent risk factor for OS and DFS of HCC (P<0.05). Conclusion: ZNF207 expression is elevated in HCC and associated with adverse clinicopathological factors, indicating poor prognosis for HCC.