依达拉奉对缺血性脑卒中大鼠模型脑缺血区炎症因子及p38MAPK、p42MAPK、p44MAPK蛋白表达的影响

Effects of Edaravone on Inflammatory Factors and Protein Expression of p38MAPK,p42MAPK and p44MAPK in Cerebral Ischemic Area of Ischemic Stroke Rat Model

【目的】探讨依达拉奉对缺血性脑卒中(CIS)大鼠模型脑缺血区炎症因子TNF-a和IL-6及p38MAPK、p42MAPK、p44MAPK蛋白表达的影响。【方法】80只大鼠随机分为生理盐水组(40只)和依达拉奉组(40只),依达拉奉组腹腔注射依达拉奉5mg/(kg·d),共注射1周,生理盐水组腹腔注射等量生理盐水。给药1周后依达拉奉组、生理盐水组分别随机分为依达拉奉假手术组(20只)和依达拉奉模型组(20只)及生理盐水假手术组(20只)和生理盐水模型组(20只)。采用线栓法制备CIS大鼠模型,ELISA法检测大鼠模型脑缺血区炎症因子TNF-α和IL-6,Western blot检测大鼠模型脑缺血区p38MAPK、p42MAPK、p44MAPK蛋白并比较。【结果】生理盐水模型组、依达拉奉模型组大鼠脑缺血区组织中TNF-α、IL-6及p38MAPK蛋白表达水平显著高于生理盐水假手术组和依达拉奉假手术组,且生理盐水模型组大鼠脑缺血区组织中TNF-α、IL-6及p38MAPK蛋白表达水平显著高于依达拉奉模型组,其差异均具有统计学意义(P<0.05),但各组大鼠p42MAPK、p44MAPK蛋白表达水平比较差异无统计学意义(P>0.05)。【结论】依达拉奉能抑制CIS大鼠脑缺血区炎症反应,其可能通过p38MAPK途径抑制缺血区炎症反应。

[Objective] To investigate the effects of edaravone on the expression of inflammatory factors TNF-alpha,IL6p,p38MAPK,p42MAPK and p44MAPK in cerebral ischemia of ischemic stroke(CIS)rat model.[Methods]Eighty rats were randomly divided into saline group(40 rats)and edaravone group(40 rats).The edaravone group was intraperitoneally injected with 5 mg/(kg d)edaravone for one week.The saline group was intraperitoneally injected with the same amount of saline.One week after administration,the edaravone group and the saline group were randomly dividedinto edaravone sham operation group(20 rats),edaravone model group(20 rats),saline sham operation group(20 rats)and saline model group(20 rats).CIS rat model was established by thread embolization.Inflammatory factors TNF-alpha and IL-6 were detected by ELISA.Western blot was used to detect p38MAPK,p42MAPK and p44MAPK proteins in cerebral ischemic area of rats.[Results]The expression levels of TNF-alpha,Il-6 and p38MAPK protein in cerebral ischemic tissue of rats in the saline model group and the edaravone model group were significantly higher than those in the saline sham operation group and the edaravone sham operation group,and the expression levels of TNF-alpha,IL-6 and p38MAPK protein in cerebral ischemic tissue of rats in the saline model group were significantly higher than those in the edaravone model group(P<0.05).However,there was no significant difference in the protein expression levels of p42MAPK and p44MAPK between the groups(P>0.05).[Conclusion]Edaravone can inhibit the inflammatory response in cerebral ischemic area of CIS rats,which may inhibit the inflammatory response in ischemic area through p38MAPK pathway.