不同物种雄性鸣声刺激下雌性凹耳蛙脑组织中ZENK的表达

The Expression of ZENK in Brain Tissue of Female Concave-eared Torrent Frogs(Odorrana tormota) under Male Vocal Stimulation of Different Species

通过免疫组化SABC法,研究在背景噪音刺激、雄性凹耳蛙(Odorrana tormota)声音刺激以及雄性大绿臭蛙(O. graminea)声音刺激下ZENK蛋白在雌性凹耳蛙脑部的表达。结果显示,上述三种声音刺激下雌性凹耳蛙的端脑、间脑和小脑区域均未发现ZENK蛋白的阳性细胞。在雄性凹耳蛙声音刺激下,雌性凹耳蛙中脑的视顶盖、脚间核、中脑深部核、被盖、半环隆枕以及延脑的孤束核、中缝核、网状核、网状结构、上橄榄核部位均有阳性细胞分布,在大绿臭蛙声音刺激下,这些部位的阳性细胞数量明显减少。本实验中ZENK蛋白阳性细胞表达部位的一部分是位于与两栖类处理听觉信息有关的中脑半环隆枕、被盖以及延脑的上橄榄核,由此可推断,在雌性凹耳蛙ZENK蛋白与听觉信息处理有着密不可分的联系。

Immunohistochemical SABC was performed to analyze the expression of ZENK protein in the brain of female concave-eared torrent frogs(Odorrana tomorta)under background noise,the voice of male concave-eared torrent frogs and the voice of the male large odorous frog(O.graminea).The results showed that there were no ZENK protein-positive cells in the telencephalon,diencephalon and cerebellum of the female concave-eared torrent frogs(Fig.2,4)stimulated by aforementioned three voices.The cells in optic tectum,interpeduncular nucleus rostral portion,nucleus profundus mesencephali,tegmentum,torus semicircularis of the mesencephalon and the cells in solit tract nucleus,raphe nuclei,reticular nuclei,reticular formation,superior olivary nucleus of the medulla oblongata in female concave-eared torrent frogs showed positive reaction under the stimulation by the voice of male concave-eared torrent frogs(Fig.3,5),while the numbers of positive cells in aforementioned areas of the brain were markedly reduced under the stimulation from the voice of the large odorous frog.Part of the ZENK protein-positive cells were located in the torus semicircularis,tegmentum of mesencephalon and the superior olive nucleus of medulla oblongata,which were related to the processing of auditory information in amphibians.It can be inferred that ZENK protein is closely related to the processing of auditory information in female concave-eared torrent frogs.