滤泡辅助性T细胞在肝棘球蚴病患者外周血中的表达

Expression of follicular helper T cells in peripheral blood of patients with hepatic echinococcosis

目的检测肝棘球蚴病患者和健康对照者外周血中滤泡辅助性T细胞(Tfh)和白细胞介素-21(IL-21)表达水平,探讨它们与肝棘球蚴病进展的关系。方法收集青海省人民医院肝棘球蚴病患者和健康体检者各50例,采用流式细胞术检测和比较肝棘球蚴病患者和健康对照者外周血中Tfh细胞表达水平,采用酶联免疫吸附试验检测和比较肝棘球蚴病患者和健康对照者血清中IL-21水平,分析肝棘球蚴病患者外周血Tfh细胞表达水平和血清IL-21含量之间的相关性。结果肝棘球蚴病患者外周血中CD4+CXCR5+T细胞(18.49%±5.67%vs. 16.18%±4.04%,P<0.05)、CD4+CXCR5+PD-1+T细胞(4.94%±1.91%vs. 2.29%±0.79%,P<0.05)和CD4+CRCR5+ICOS+PD-1+T细胞比例(30.93%±24.10%vs.21.07%±14.25%,P<0.05)均显著高于健康对照者,但肝棘球蚴病患者和健康对照者外周血CD4+CRCR5+ICOS+T细胞比例差异无统计学意义(0.29%±0.32%vs. 0.25%±0.31%,P> 0.05)。肝棘球蚴病患者血清IL-21含量显著高于健康对照者([293.35±2 03.65)pg/mL vs.(192.72±70.09)pg/mL,P<0.05],但肝棘球蚴病患者外周血Tfh细胞表达水平和血清IL-21含量无相关性(P> 0.05)。结论肝棘球蚴病患者外周血Tfh细胞及血清IL-21表达水平升高,Tfh细胞和IL-21可能参与了肝棘球蚴病进展。

Objective To detect the expression of follicuLar helper T cells (Tfh) and interleukin-21 (IL-21) in the peripheral blood of patients with hepatic echinococcosis and healthy controls, so as to explore the associations of Tfli and IL-21 expression with the progression of hepatic echinococcosis. Methods Fifty cases of hepatic echinococcosis and healthy controls were collected from Qinghai Provincial People's Hospital, respectively. Flow cytometry was used to detect the expression of Tfh cells in the peripheral blood of hepatic echinococcosis patients and healthy controls , and enzyme-linked immunosorbent assay (ELISA )was used to detect serum IL-21 expression in hepatic echinococcosis patients and healthy controls. The correlation between Tfh cell expression and serum IL-21 level was examined in the patients with hepatic echinococcosis. Results Flow cytometry detected a higher percentage of CD4^+CXCR5^+ICOS^+PD-1^+T cells (1&49%±5.67% vs. 16.18%±4.04%, P < 0.05), CD4^+CXCR5^+ICOS^+PD-1^+ T cells (4.94%±1.91% vs. 2.29%±0.79%, P < 0.05) and CD4^+CXCR5^+ICOS^+PD-1^+ T cells (30.93%±24.10% vs. 21.07%±14.25%, P < 0.05) in hepatic echinococcosis patients than in healthy controls, and no significant difference was seen in the percentage of CD4XRCR5 1COS+ T cells between the patients and controls (0.29%±0.32% vs. 0.25%±0.31%, P > 0.05). The serum IL-21 level was significantly higher in the patients with hepatic echinococcosis than in healthy controls [(293.35±2 03.65 ) pg/mL vs.(192.72±70.09) pg/mL, P<0.05];however, there was no correlation between the Tfh cell expression and serum IL- 21 level in patients with hepatic echinococcosis (P>0.05 ). Conclusion The expression of peripheral blood Tfh cells and serum IL-21 is elevated in patients with hepatic echinococcosis, and Tfh cells and IL-21 may contribute to the progression of hepatic echinococcosis.