超临界CO_2萃取汉麻3种大麻酚工艺及其抑菌性研究

Supercritical CO_2 Extraction Technology and Antibacterial Effect of Three Cannabinoids in Hemp

建立了超临界CO_2萃取汉麻3种大麻酚工艺,考察了其萃取物抑菌性能。通过相应单因素与正交试验分析,确定了超临界CO_2萃取汉麻3种大麻酚的最佳工艺:萃取温度45℃、萃取时间70 min、夹带剂用量300 m L、萃取次数3次。对大肠杆菌与金黄色葡萄球菌MIC分别为8 mg/m L与6 mg/m L条件下进行滤纸片法抑菌试验。此条件下大麻二酚(CBD)为3. 189 mg/g,六氢大麻酚(CBN)为2. 206 mg/g,四氢大麻酚(Δ9-THC)为58. 757 mg/g,即3种大麻酚为64. 152mg/g。大肠杆菌与金黄色葡萄球菌抑菌圈直径分别为15. 4 mm与25. 8 mm,此超临界萃取物具有一定的抑菌性能。

To establish a supercritical CO2 extraction method for three cannabinoids in hemp and investigate the antimicrobial activity.The supercritical CO2 extraction method for three cannabinoids in hemp was established based on the analysis of the single factor and orthogonal tests.Three cannabinoids in hemp are extracted at extraction temperature 45℃,extraction time 70 min,entrainment and dosage 300 mL and extraction times for 3.The filter method for bacteriostatic test was established with the MIC of escherichia coli and staphylococcus aureus at 8 mg/mL and 6 mg/mL.By this way,CBD content was 3.189 mg/g,CBN content was 2.206 mg/g,Δ9-THC content was 58.757 mg/g,so three cannabinoids content was 64.152 mg/g.The bacteriostatic rings of escherichia coli and staphylococcus aureus were 15.4 mm and 25.8 mm.The supercritical extract had antibacterial effect.