3D打印气管的孔径遴选、表面修饰与性能评价

Selection of the appropriate pore size, surface modification technique for 3D printed tracheal graft and evaluation of its property

目的通过3D打印技术制备聚己内酯(PCL)多孔气管支架,评价其生物力学性能;遴选适宜的孔径大小及表面修饰方法,为体内气管移植寻求更合适的支架材料。方法利用3D打印技术制备PCL多孔气管支架并采用纵向拉伸、径向压缩和三点弯曲试验评价其生物力学性能;通过水解、胺化和纳米二氧化硅修饰对支架材料进行表面改性,能谱分析评价其表面修饰效果;通过CCK-8、电镜扫描观察等方法评估修饰后材料的生物相容性及对细胞黏附、增殖行为的影响。结果3D打印PCL气管支架具有与兔新鲜气管相似的形态结构(P>0.05),生物力学性能更优(P<0.05);气管支架孔径大小为200μm更适合细胞的黏附与增殖(P<0.05);与水解、胺化的修饰方法相比,纳米二氧化硅修饰后的支架亲细胞性明显改善,更利于细胞黏附与增殖(P<0.05).结论3D打印PCL气管支架具有良好的生物力学性能,管壁适宜孔径大小为200μm,纳米二氧化硅修饰为适宜的表面修饰方法。

Objective To prepare 3D printed porous tracheal graft fabricated by PCL and to select the appropriate pore size and surface modificiilion techniques, in order to explore its effect on cell behavior. Methods The PCL porous tracheal graft Has prepared by 3D printing lechnology and biomechanical properties of the graft were measured by means of longitudinal tension , radial compression and three-point bending test. The porous grafts were surface-modified through hydrolysis, amination and nanocnstcillization treatnirnt and then characterized by energy dispersive spectroscopy( EDS). The effect of differenl pore sizes and surface modifications on the cell proliferation behavior was evaluated by CCK-8 and scanning electron microscopy (SEM ). Results The 3D printed porous tracheal gnift luid similar morphology wilh the native liacheas( P > 0. 05 ) and belter biomechanical properties( P <0.05 ). It was more suitable for cell adhesion and proliferation when the pore size is 200 μm (P <0. 05 ). Compared to hydrolysis and amination , nanocry stallization treatment successfully improved the cytotropism of the 3D printed tracheal graft( P <0. 05 ). Conclusion 3D printed porous tracheal graft shows favorable biomechanical properties.The appropriate pore size of the 3D printed porous tracheal graft is 200 μm and the appropriate surface modification techniques is nanocnslallization.